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Farnesylation mediates brassinosteroid biosynthesis to regulate abscisic acid responses
journal contribution
posted on 2023-05-19, 02:29 authored by Northey, JGB, Liang, S, Jamshed, M, Deb, S, Eloise FooEloise Foo, James ReidJames Reid, McCourt, P, Samuel, MAProtein farnesylation is a post-translational modification involving the addition of a 15-carbon farnesyl isoprenoid to the carboxy terminus of select proteins1–3. Although the roles of this lipid modification are clear in both fungal and animal signalling, many of the mechanistic functions of farnesylation in plant signalling are still unknown. Here, we show that CYP85A2, the cytochrome P450 enzyme that performs the last step in brassinosteroid biosynthesis (conversion of castasterone to brassinolide)4, must be farnesylated to function in Arabidopsis. Loss of either CYP85A2 or CYP85A2 farnesylation results in reduced brassinolide accumulation and increased plant responsiveness to the hormone abscisic acid (ABA) and overall drought tolerance, explaining previous observations5. This result not only directly links farnesylation to brassinosteroid biosynthesis but also suggests new strategies to maintain crop yield under challenging climatic conditions.
Funding
Australian Research Council
History
Publication title
Nature PlantsIssue
8Article number
16114Number
16114Pagination
1-8ISSN
2055-026XDepartment/School
School of Natural SciencesPublisher
Nature Publishing GroupPlace of publication
United KingdomRights statement
Copyright 2016 Nature plantsRepository Status
- Restricted