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Expression of sucrose synthase in the developing endosperm is essential for early seed development in cotton


Ruan, Y-L and Llewellyn, DJ and Liu, Q and Xu, S-M and Wu, L-M and Wang, L and Furbank, RT, Expression of sucrose synthase in the developing endosperm is essential for early seed development in cotton, Functional Plant Biology, 35, (5) pp. 382-393. ISSN 1445-4408 (2008) [Refereed Article]

Copyright Statement

Copyright 2008 CSIRO

DOI: doi:10.1071/FP08017


Successful seed development requires coordinated interaction of the endosperm and embryo. In most dicotyledonous seeds, the endosperm is crushed and absorbed by the expanding embryo in the later stages of seed development. Little is known about the metabolic interaction between the two filial tissues early in seed development. We examined the potential role of sucrose synthase (Sus) in the endosperm development of cotton. Sus was immunologically localised in the cellularising endosperm, but not in the heart-stage embryo at 10 days after anthesis. The activities of Sus and acid invertase were significantly higher in the endosperm than in the young embryos, which corresponded to a steep concentration difference in hexoses between the endosperm and the embryo. This observation indicates a role for the endosperm in generating hexoses for the development of the two filial tissues. Interestingly, Sus expression and starch deposition were spatially separated in the seeds. Silencing the expression of Sus in the endosperm using an RNAi approach led to the arrest of early seed development. Histochemical analyses revealed a significant reduction in cellulose and callose in the deformed endosperm cells of the Sus-suppressed seed. The data indicate a critical role of Sus in early seed development through regulation of endosperm formation.

Item Details

Item Type:Refereed Article
Keywords:cotton fibre, sugar metabolism
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Harvesting and packaging of plant products
Objective Field:Cotton lint and cotton seed
UTAS Author:Wang, L (Dr Lu Wang)
ID Code:114765
Year Published:2008
Web of Science® Times Cited:39
Deposited By:Plant Science
Deposited On:2017-02-26
Last Modified:2017-05-12

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