eCite Digital Repository

Evidence that high activity of vacuolar invertase Is required for cotton fiber and Arabidopsis root elongation through osmotic dependent and independent pathways, respectively

Citation

Wang, L and Li, X-R and Lian, H and Ni, D-A and He, Y-K and Chen, X-Y and Ruan, Y-L, Evidence that high activity of vacuolar invertase Is required for cotton fiber and Arabidopsis root elongation through osmotic dependent and independent pathways, respectively, Plant Physiology, 154, (2) pp. 744-756. ISSN 0032-0889 (2010) [Refereed Article]

Copyright Statement

Copyright 2010 American Society of Plant Biologists

DOI: doi:10.1104/pp.110.162487

Abstract

Vacuolar invertase (VIN) has long been considered as a major player in cell expansion. However, direct evidence for this view is lacking due, in part, to the complexity of multicellular plant tissues. Here, we used cotton (Gossypium spp.) fibers, fast-growing single-celled seed trichomes, to address this issue. VIN activity in elongating fibers was approximately 4-6-fold higher than that in leaves, stems, and roots. It was undetectable in fiberless cotton seed epidermis but became evident in initiating fibers and remained high during their fast elongation and dropped when elongation slowed. Furthermore, a genotype with faster fiber elongation had significantly higher fiber VIN activity and hexose levels than a slow-elongating genotype. By contrast, cell wall or cytoplasmic invertase activities did not show correlation with fiber elongation. To unravel the molecular basis of VIN-mediated fiber elongation, we cloned GhVIN1, which displayed VIN sequence features and localized to the vacuole. Once introduced to Arabidopsis (Arabidopsis thaliana), GhVIN1 complemented the short-root phenotype of a VIN T-DNA mutant and enhanced the elongation of root cells in the wild type. This demonstrates that GhVIN1 functions as VIN in vivo. In cotton fiber, GhVIN1 expression level matched closely with VIN activity and fiber elongation rate. Indeed, transformation of cotton fiber with GhVIN1 RNA interference or overexpression constructs reduced or enhanced fiber elongation, respectively. Together, these analyses provide evidence on the role of VIN in cotton fiber elongation mediated by GhVIN1. Based on the relative contributions of sugars to sap osmolality in cotton fiber and Arabidopsis root, we conclude that VIN regulates their elongation in an osmotic dependent and independent manner, respectively.

Item Details

Item Type:Refereed Article
Keywords:vacuolar invertase, cotton fiber, sugar metabolism, cell expansion
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Harvesting and Packing of Plant Products
Objective Field:Cotton Lint and Cotton Seed
Author:Wang, L (Dr Lu Wang)
ID Code:114761
Year Published:2010
Web of Science® Times Cited:62
Deposited By:Plant Science
Deposited On:2017-02-26
Last Modified:2017-05-02
Downloads:0

Repository Staff Only: item control page