eCite Digital Repository

Silencing the vacuolar invertase gene GhVIN1 blocks cotton fiber initiation from the ovule epidermis, probably by suppressing a cohort of regulatory genes via sugar signaling

Citation

Wang, L and Cook, A and Patrick, JW and Chen, X-Y and Ruan, Y-L, Silencing the vacuolar invertase gene GhVIN1 blocks cotton fiber initiation from the ovule epidermis, probably by suppressing a cohort of regulatory genes via sugar signaling, The Plant Journal, 78, (4) pp. 686-696. ISSN 0960-7412 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 The Authors The Plant Journal copyright 2014 John Wiley & Sons Ltd

DOI: doi:10.1111/tpj.12512

Abstract

Cotton fibers, the most important source of cellulose for the global textile industry, are single-celled trichomes derived from the ovule epidermis at or just prior to anthesis. Despite progress in understanding cotton fiber elongation and cell-wall biosynthesis, knowledge regarding the molecular basis of fiber cell initiation, the first step of fiber development determining the fiber yield potential, remains elusive. Here, we provide evidence that expression of a vacuolar invertase (VIN) is an early event that is essential for cotton fiber initiation. RNAi-mediated suppression of GhVIN1, a major VIN gene that is highly expressed in wild-type fiber initials, resulted in significant reduction of VIN activity and consequently a fiberless seed phenotype in a dosage dependent manner. The absence of a negative effect on seed development in these fiberless seeds indicates that the phenotype is unlikely to be due to lack of carbon nutrient. Gene expression analyses coupled with in vitro ovule culture experiments revealed that GhVIN1-derived hexose signaling may play an indispensable role in cotton fiber initiation, probably by regulating the transcription of several MYB transcription factors and auxin signaling components that were previously identified as required for fiber initiation. Together, the data represent a significant advance in understanding the mechanisms of cotton fiber initiation, and provide the first indication that VIN-mediated hexose signaling may act as an early event modulating the expression of regulatory genes and hence cell differentiation from the ovule epidermis.

Item Details

Item Type:Refereed Article
Keywords:cotton fibre, invertase, sugar signaling, MYB, auxin
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Industrial Crops
Objective Field:Sugar
Author:Wang, L (Dr Lu Wang)
ID Code:114756
Year Published:2014
Web of Science® Times Cited:16
Deposited By:Plant Science
Deposited On:2017-02-26
Last Modified:2017-11-02
Downloads:0

Repository Staff Only: item control page