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Insight into the Genome of Brochothrix thermosphacta, a Problematic Meat Spoilage Bacterium

Citation

Stanborough, T and Fegan, N and Powell, SM and Tamplin, M and Chandry, PS, Insight into the Genome of Brochothrix thermosphacta, a Problematic Meat Spoilage Bacterium, Applied and Environmental Microbiology, 83, (5) Article e02786-16. ISSN 1098-5336 (2017) [Refereed Article]

Copyright Statement

Copyright 2016 American Society for Microbiology

DOI: doi:10.1128/AEM.02786-16

Abstract

Brochothrix thermosphacta is a dominant, but poorly studied meat spoilage organism. It is a close relative of the foodborne pathogen Listeria monocytogenes and constitutes the second genera in the Listeriaceae family. Here, the genomes of 12 B. thermosphacta strains were sequenced, assembled into draft genomes, characterized and compared with the genomes of Brochothrix campestris and L. monocytogenes. Phenotypic properties including biogenic amine production, and antibiotic and heavy metal susceptibility were tested.

Comparative genomic analyses revealed a high degree of similarity among the B. thermosphacta strains, with bacteriophage genes constituting a significant proportion of the accessory genome. Genes for the production of malodourous compounds acetate, acetoin, butanediol and fatty acids were found, as were stress response regulatory genes, which likely play important roles in the spoilage process. Amino acid decarboxylases were not identified in the genomes and phenotypic testing confirmed their absence. Orthologs of Listeria virulence proteins involved in virulence regulation, intracellular survival and surface protein anchoring were found, however, key virulence genes were absent. Analysis of antibiotic susceptibility showed strains were sensitive to the four tested antibiotics, except for one tetracycline resistant isolate with plasmid-mediated tetracycline resistance genes. Strains tolerated higher levels of copper and cobalt than of cadmium, although not at concentrations high enough to categorize the strains as resistant.

This study provides insight into the Brochothrix genome, links previous phenotypic data and data provided here to gene inventory and identifies genes that may contribute to the persistence of this organism in the food chain.

Item Details

Item Type:Refereed Article
Keywords:meat spoilage
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Microbiology not elsewhere classified
Objective Division:Animal Production and Animal Primary Products
Objective Group:Other Animal Production and Animal Primary Products
Objective Field:Animal Production and Animal Primary Products not elsewhere classified
Author:Powell, SM (Dr Shane Powell)
Author:Tamplin, M (Professor Mark Tamplin)
ID Code:114375
Year Published:2017 (online first 2016)
Deposited By:TIA - Research Institute
Deposited On:2017-02-14
Last Modified:2017-08-18
Downloads:0

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