File(s) under permanent embargo
Assaying germination and seedling responses of Arabidopsis to karrikins
Karrikins are a small family of naturally occurring plant growth regulators present in the smoke and char produced from burning plant material in wildfires. They can stimulate germination of dormant seed and can influence seedling morphogenesis. Although Arabidopsis thaliana is not considered to be a smoke-responsive species, karrikins will stimulate seed germination under the appropriate circumstances and will cause repression of hypocotyl elongation in low light. This chapter describes how to conduct assays of the activity of karrikins on Arabidopsis seeds and seedlings. The methods presented can potentially be modified for use in a range of Arabidopsis genotypes or in other plant species.
History
Publication title
Plant Hormones: Methods and ProtocolsVolume
1497Editors
J Kleine-Vehn & M SauerPagination
29-36ISBN
9781493964673Department/School
School of Natural SciencesPublisher
Springer New YorkPlace of publication
New YorkExtent
22Rights statement
Copyright 2017 Springer Science+Business Media New YorkRepository Status
- Restricted