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Assaying germination and seedling responses of Arabidopsis to karrikins

Citation

Waters, MT and Flematti, GR and Smith, SM, Assaying germination and seedling responses of Arabidopsis to karrikins, Plant Hormones: Methods and Protocols, Springer New York, J Kleine-Vehn & M Sauer (ed), New York, pp. 29-36. ISBN 978-1-4939-6467-3 (2017) [Research Book Chapter]

DOI: doi:10.1007/978-1-4939-6469-7_4

Abstract

Karrikins are a small family of naturally occurring plant growth regulators present in the smoke and char produced from burning plant material in wildfires. They can stimulate germination of dormant seed and can influence seedling morphogenesis. Although Arabidopsis thaliana is not considered to be a smoke-responsive species, karrikins will stimulate seed germination under the appropriate circumstances and will cause repression of hypocotyl elongation in low light. This chapter describes how to conduct assays of the activity of karrikins on Arabidopsis seeds and seedlings. The methods presented can potentially be modified for use in a range of Arabidopsis genotypes or in other plant species.

Item Details

Item Type:Research Book Chapter
Keywords:karrikins, Arabidopsis thaliana, seed germination, dormancy, hypocotyl elongation, photomorphogenesis
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Smith, SM (Professor Steven Smith)
ID Code:114365
Year Published:2017
Deposited By:Plant Science
Deposited On:2017-02-14
Last Modified:2017-02-14
Downloads:0

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