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Cell-type-specific H+-ATPase activity in root tissues enables K+ retention and mediates acclimation of barley (Hordeum vulgare) to salinity stress


Shabala, L and Zhang, J and Pottosin, I and Bose, J and Zhu, M and Fuglsang, AT and Velarde-Buendia, A and Massart, A and Hill, CB and Roessner, U and Bacic, A and Wu, H and Azzarello, E and Pandolfi, C and Zhou, M and Poschenrieder, C and Mancuso, S and Shabala, S, Cell-type-specific H+-ATPase activity in root tissues enables K+ retention and mediates acclimation of barley (Hordeum vulgare) to salinity stress, Plant Physiology, 172, (4) pp. 2445-2458. ISSN 1532-2548 (2016) [Refereed Article]

Copyright Statement

Copyright 2016 American Society of Plant Biologists

DOI: doi:10.1104/pp.16.01347


While the importance of cell type specificity in plant adaptive responses is widely accepted, only a limited number of studies have addressed this issue at the functional level. We have combined electrophysiological, imaging, and biochemical techniques to reveal the physiological mechanisms conferring higher sensitivity of apical root cells to salinity in barley (Hordeum vulgare). We show that salinity application to the root apex arrests root growth in a highly tissue- and treatment-specific manner. Although salinity-induced transient net Na+ uptake was about 4-fold higher in the root apex compared with the mature zone, mature root cells accumulated more cytosolic and vacuolar Na+, suggesting that the higher sensitivity of apical cells to salt is not related to either enhanced Na+ exclusion or sequestration inside the root. Rather, the above differential sensitivity between the two zones originates from a 10-fold difference in K+ efflux between the mature zone and the apical region (much poorer in the root apex) of the root. Major factors contributing to this poor K+ retention ability are (1) an intrinsically lower H+-ATPase activity in the root apex, (2) greater salt-induced membrane depolarization, and (3) a higher reactive oxygen species production under NaCl and a larger density of reactive oxygen species-activated cation currents in the apex. Salinity treatment increased (2- to 5-fold) the content of 10 (out of 25 detected) amino acids in the root apex but not in the mature zone and changed the organic acid and sugar contents. The causal link between the observed changes in the root metabolic profile and the regulation of transporter activity is discussed.

Item Details

Item Type:Refereed Article
Keywords:barley, abiotic stress tolerance, drought
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Crop and pasture production
Research Field:Crop and pasture improvement (incl. selection and breeding)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Grains and seeds
Objective Field:Barley
UTAS Author:Shabala, L (Associate Professor Lana Shabala)
UTAS Author:Zhang, J (Miss Jingyi Zhang)
UTAS Author:Pottosin, I (Professor Igor Pottosin)
UTAS Author:Bose, J (Dr Jayakumar Bose)
UTAS Author:Zhu, M (Ms Min Zhu)
UTAS Author:Wu, H (Mr Honghong Wu)
UTAS Author:Zhou, M (Professor Meixue Zhou)
UTAS Author:Shabala, S (Professor Sergey Shabala)
ID Code:114281
Year Published:2016
Web of Science® Times Cited:111
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2017-02-09
Last Modified:2017-11-06

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