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A novel Zea mays ssp. mexicana L. MYC-type ICE-like transcription factor gene ZmmICE1, enhances freezing tolerance in transgenic Arabidopsis thaliana

Citation

Lu, X and Yang, D and Yu, M and Lai, J and Wang, C and McNeil, D and Zhou, M and Yang, C, A novel Zea mays ssp. mexicana L. MYC-type ICE-like transcription factor gene ZmmICE1, enhances freezing tolerance in transgenic Arabidopsis thaliana, Plant Physiology and Biochemistry, 113 pp. 78-88. ISSN 0981-9428 (2017) [Refereed Article]

Copyright Statement

Copyright 2017 Elsevier Masson SAS

DOI: doi:10.1016/j.plaphy.2017.02.002

Abstract

The annual Zea mays ssp. mexicana L., a member of the teosinte group, is a close wild relative of maize and thus can be effectively used in maize improvement. In this study, an ICE-like gene, ZmmICE1, was isolated from a cDNA library of RNA-Seq from cold-treated seedling tissues of Zea mays ssp. mexicana L. The deduced protein of ZmmICE1 contains a highly conserved basic helix-loop-helix (bHLH) domain and C-terminal region of ICE-like proteins. The ZmmICE1 protein localizes to the nucleus and shows sumoylation when expressed in an Escherichia coli reconstitution system. In addition, yeast one hybrid assays indicated that ZmmICE1 has transactivation activities. Moreover, ectopic expression of ZmmICE1 in the Arabidopsis ice1-2 mutant increased freezing tolerance. The ZmmICE1 overexpressed plants showed lower electrolyte leakage (EL), reduced contents of malondialdehyde (MDA). The expression of downstream cold related genes of Arabidopsis C-repeat-binding factors (AtCBF1, AtCBF2 and AtCBF3), cold-responsive genes (AtCOR15A and AtCOR47), kinesin-1 member gene (AtKIN1) and responsive to desiccation gene (AtRD29A) was significantly induced when compared with wild type under low temperature treatment. Taken together, these results indicated that ZmmICE1 is the homolog of Arabidopsis inducer of CBF expression genes (AtICE1/2) and plays an important role in the regulation of freezing stress response.

Item Details

Item Type:Refereed Article
Keywords:ZmmICE1, transcription factor, transgenic Arabidopsis thaliana, freezing tolerance, Zea mays ssp. mexicana L., Zea Mays ssp. maxicana L., frost tolerance
Research Division:Agricultural and Veterinary Sciences
Research Group:Crop and Pasture Production
Research Field:Crop and Pasture Improvement (Selection and Breeding)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Other Plant Production and Plant Primary Products
Objective Field:Forest Product Traceability and Quality Assurance
Author:Lu, X (Mr Xiang Lu)
Author:McNeil, D (Professor David McNeil)
Author:Zhou, M (Associate Professor Meixue Zhou)
ID Code:114279
Year Published:2017
Web of Science® Times Cited:1
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2017-02-09
Last Modified:2017-11-16
Downloads:0

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