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RNA-seq analysis of cold and drought responsive transcriptomes of Zea mays ssp. mexicana L

Citation

Lu, X and Zhou, X and Cao, Y and Zhou, M and McNeil, D and Liang, S and Yang, C, RNA-seq analysis of cold and drought responsive transcriptomes of Zea mays ssp. mexicana L, Frontiers in Plant Science, 8 Article 136. ISSN 1664-462X (2017) [Refereed Article]


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Copyright Statement

Copyright 2017 Lu, Zhou, Cao, Zhou, McNeil, Liang and Yang. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.3389/fpls.2017.00136

Abstract

The annual Zea mays ssp. mexicana L. is a member of teosinte, a wild relative of the Zea mays spp. mays L. This subspecies has strong growth and regeneration ability, high tiller numbers, high protein and lysine content as well as resistance to many fungal diseases, and it can be effectively used in maize improvement. In this study, we reported a Zea mays ssp. mexicana L. transcriptome by merging data from untreated control (CK), cold (4C) and drought (PEG2000, 20%) treated plant samples. A total of 251,145 transcripts (N50 = 1,269 bp) and 184,280 unigenes (N50 = 923 bp) were predicted, which code for homologs of near 47% of the published maize proteome. Under cold conditions, 2,232 and 817 genes were up-regulated and down-regulated, respectively, while fewer genes were up-regulated (532) and down-regulated (82) under drought stress, indicating that Zea mays ssp. mexicana L. is more sensitive to the applied cold rather than to the applied drought stresses. Functional enrichment analyses identified many common or specific biological processes and gene sets in response to drought and cold stresses. The ABA dependent pathway, trehalose synthetic pathway and the ICE1-CBF pathway were up-regulated by both stresses. GA associated genes have been shown to differentially regulate the responses to cold in close subspecies in Zea mays. These findings and the identified functional genes can provide useful clues for improving abiotic stress tolerance of maize.

Item Details

Item Type:Refereed Article
Keywords:Zea mays ssp. mexicana L., cold tolerance, drought tolerance, transcriptome, differentially expressed genes, barley, abiotic stress tolerance
Research Division:Agricultural and Veterinary Sciences
Research Group:Crop and Pasture Production
Research Field:Crop and Pasture Improvement (Selection and Breeding)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Winter Grains and Oilseeds
Objective Field:Barley
Author:Lu, X (Mr Xiang Lu)
Author:Zhou, M (Associate Professor Meixue Zhou)
Author:McNeil, D (Professor David McNeil)
ID Code:114278
Year Published:2017
Web of Science® Times Cited:2
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2017-02-09
Last Modified:2017-11-16
Downloads:7 View Download Statistics

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