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Diverse microbial communities in non-aerated compost teas suppress bacterial wilt


Mengesha, WK and Powell, SM and Evans, KJ and Barry, KM, Diverse microbial communities in non-aerated compost teas suppress bacterial wilt, World Journal of Microbiology and Biotechnology, 33 Article 49. ISSN 0959-3993 (2017) [Refereed Article]

Copyright Statement

Springer Science+Business Media Dordrecht 2017

DOI: doi:10.1007/s11274-017-2212-y


Non-aerated compost teas (NCTs) are water extracts of composted organic materials and are used to suppress soil borne and foliar disease in many pathosystems. Greenhouse trials were used to test the effectiveness of NCTs to suppress potato bacterial wilt caused by Ralstonia solanacearum on plants grown in soils inoculated with a virulent isolate of the pathogen (biovar II). NCTs prepared from matured compost sources: agricultural waste (AWCT), vermicompost (VCT) and solid municipal waste (SMWCT) were evaluated at three initial application times (7 days before inoculation, at time of inoculation and 7 days after inoculation) prior to weekly applications, in a randomized complete-block design. AWCT applied initially at the time of inoculation resulted in the greatest disease suppression, with the disease severity index 2.5-fold less than the non-treated plants and the "area under the disease progress curve" (AUDPC) 3.2-fold less. VCT and SMWCT were less suppressive than AWCT regardless of initial application time. Next generation sequencing of the v4 region of 16S rRNA gene and the internal transcribed spacer region (ITS1) revealed that diversity and composition of the bacterial and fungal communities across the NCTs varied significantly. Dominant bacterial phyla such as Actinobacteria, Bacteroidetes, Firmicutes, Proteobacteria, Verrucomicrobia, Chloroflexi, Planctomycetes, Acidobacteria, and a fungal phylum Ascomycota were detected in all NCTs. AWCT had optimum physico-chemical measurements with higher bacterial Shannon diversity indices (H) and fungal richness (S) than the other treatments. We conclude that bacterial wilt of potatoes grown in controlled conditions can be suppressed by a non-aerated compost tea with a high microbial diversity when applied at planting and weekly thereafter.

Item Details

Item Type:Refereed Article
Keywords:potato, Ralstonia, bacterial wilt, compost tea, crop protection, disease severity, next generation sequencing, microbial diversity
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Horticultural production
Research Field:Horticultural crop protection (incl. pests, diseases and weeds)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Horticultural crops
Objective Field:Field grown vegetable crops
UTAS Author:Mengesha, WK (Mr Wossen Mengesha)
UTAS Author:Powell, SM (Dr Shane Powell)
UTAS Author:Evans, KJ (Professor Katherine Evans)
UTAS Author:Barry, KM (Associate Professor Kara Barry)
ID Code:114237
Year Published:2017
Web of Science® Times Cited:18
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2017-02-09
Last Modified:2018-05-22

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