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Electrokinetic Properties of Lubricin Antiadhesive Coatings in Microfluidic Systems
journal contribution
posted on 2023-05-19, 00:45 authored by Greene, GW, Duffy, E, Shallan, A, Wuethrich, A, Brett PaullBrett PaullLubricin is a glycoprotein found in articular joints which has long been recognized as being an important biological boundary lubricant molecule and, more recently, an impressive antiadhesive that readily self-assembles into a well ordered, polymer brush layer on virtually any substrate. The lubricin molecule possesses an overabundance of anionic charge, a property that is atypical among antiadhesive molecules, that enables its use as a coating for applications involving electrokinetic processes such as electrophoresis and electroosmosis. Coating the surfaces of silica and polymeric microfluidic devices with self-assembled lubricin coatings affords a unique combination of excellent fouling resistance and high charge density that enables notoriously "sticky" biomolecules such as proteins to be used and controlled electrokinetically in the device without complications arising from nonspecific adsorption. Using capillary electrophoresis, we characterized the stability, uniformity, and electrokinetic properties of lubricin coatings applied to silica and PTFE capillaries over a range of run buffer pHs and when exposed to concentrated solutions of protein. In addition, we demonstrate the effectiveness of lubricin as a coating to minimize nonspecific protein adsorption in an electrokinetically controlled polydimethylsiloxane/silica microfluidic device.
Funding
Australian Research Council
History
Publication title
LangmuirVolume
32Issue
7Pagination
1899-1908ISSN
0743-7463Department/School
School of Natural SciencesPublisher
American Chemical SocietyPlace of publication
United StatesRights statement
Copyright 2016 American Chemical SocietyRepository Status
- Restricted