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Electrokinetic Properties of Lubricin Antiadhesive Coatings in Microfluidic Systems

Citation

Greene, GW and Duffy, E and Shallan, A and Wuethrich, A and Paull, B, Electrokinetic Properties of Lubricin Antiadhesive Coatings in Microfluidic Systems, Langmuir, 32, (7) pp. 1899-1908. ISSN 0743-7463 (2016) [Refereed Article]

Copyright Statement

Copyright 2016 American Chemical Society

DOI: doi:10.1021/acs.langmuir.5b03535

Abstract

Lubricin is a glycoprotein found in articular joints which has long been recognized as being an important biological boundary lubricant molecule and, more recently, an impressive antiadhesive that readily self-assembles into a well ordered, polymer brush layer on virtually any substrate. The lubricin molecule possesses an overabundance of anionic charge, a property that is atypical among antiadhesive molecules, that enables its use as a coating for applications involving electrokinetic processes such as electrophoresis and electroosmosis. Coating the surfaces of silica and polymeric microfluidic devices with self-assembled lubricin coatings affords a unique combination of excellent fouling resistance and high charge density that enables notoriously "sticky" biomolecules such as proteins to be used and controlled electrokinetically in the device without complications arising from nonspecific adsorption. Using capillary electrophoresis, we characterized the stability, uniformity, and electrokinetic properties of lubricin coatings applied to silica and PTFE capillaries over a range of run buffer pHs and when exposed to concentrated solutions of protein. In addition, we demonstrate the effectiveness of lubricin as a coating to minimize nonspecific protein adsorption in an electrokinetically controlled polydimethylsiloxane/silica microfluidic device.

Item Details

Item Type:Refereed Article
Keywords:Electrokinetic properties; microfluidics; lubricin
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Duffy, E (Miss Emer Duffy)
Author:Shallan, A (Ms Aliaa Shallan)
Author:Wuethrich, A (Mr Alain Wuethrich)
Author:Paull, B (Professor Brett Paull)
ID Code:113613
Year Published:2016
Funding Support:Australian Research Council (CE140100012)
Web of Science® Times Cited:1
Deposited By:Chemistry
Deposited On:2017-01-09
Last Modified:2017-03-14
Downloads:0

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