eCite Digital Repository

Wall modified photonic crystal fibre capillaries as porous layer open tubular columns for in-capillary micro-extraction and capillary chromatography


Kazarian, AA and Sanz Rodriguez, E and Deverell, JA and McCord, J and Muddiman, DC and Paull, B, Wall modified photonic crystal fibre capillaries as porous layer open tubular columns for in-capillary micro-extraction and capillary chromatography, Analytica Chimica Acta, 905 pp. 1-7. ISSN 0003-2670 (2016) [Refereed Article]

Copyright Statement

Copyright 2015 Elsevier B.V.

DOI: doi:10.1016/j.aca.2015.10.005


Wall modified photonic crystal fibre capillary columns for in-capillary micro-extraction and liquid chromatographic separations is presented. Columns contained 126 internal parallel 4μm channels, each containing a wall bonded porous monolithic type polystyrene-divinylbenzene layer in open tubular column format (PLOT). Modification longitudinal homogeneity was monitored using scanning contactless conductivity detection and scanning electron microscopy. The multichannel open tubular capillary column showed channel diameter and polymer layer consistency of 4.20.1μm and 0.260.02μm respectively, and modification of 100% of the parallel channels with the monolithic polymer. The modified multi-channel capillaries were applied to the in-capillary micro-extraction of water samples. 500μL of water samples containing single μgL−1 levels of polyaromatic hydrocarbons were extracted at a flow rate of 10μLmin−1, and eluted in 50μL of acetonitrile for analysis using HPLC with fluorescence detection. HPLC LODs were 0.08, 0.02 and 0.05μgL−1 for acenaphthene, anthracene and pyrene, respectively, with extraction recoveries of between 77 and 103%. The modified capillaries were also investigated briefly for direct application to liquid chromatographic separations, with the retention and elution of a standard protein (cytochrome c) under isocratic conditions demonstrated, proving chromatographic potential of the new column format, with run-to-run retention time reproducibility of below 1%.

Item Details

Item Type:Refereed Article
Keywords:photonic crystal fibres; wall modified; micro-extraction; capillary chromatography
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Kazarian, AA (Dr Tom Kazarian)
UTAS Author:Sanz Rodriguez, E (Dr Estrella Sanz Rodriguez)
UTAS Author:Deverell, JA (Dr Jeremy Deverell)
UTAS Author:Paull, B (Professor Brett Paull)
ID Code:113612
Year Published:2016
Funding Support:Australian Research Council (DP140104323)
Web of Science® Times Cited:21
Deposited By:Chemistry
Deposited On:2017-01-09
Last Modified:2017-10-30

Repository Staff Only: item control page