eCite Digital Repository
Wall modified photonic crystal fibre capillaries as porous layer open tubular columns for in-capillary micro-extraction and capillary chromatography
Citation
Kazarian, AA and Sanz Rodriguez, E and Deverell, JA and McCord, J and Muddiman, DC and Paull, B, Wall modified photonic crystal fibre capillaries as porous layer open tubular columns for in-capillary micro-extraction and capillary chromatography, Analytica Chimica Acta, 905 pp. 1-7. ISSN 0003-2670 (2016) [Refereed Article]
Copyright Statement
Copyright 2015 Elsevier B.V.
DOI: doi:10.1016/j.aca.2015.10.005
Abstract
Wall modified photonic crystal fibre capillary columns for in-capillary micro-extraction and liquid chromatographic separations is presented. Columns contained 126 internal parallel 4 μm channels, each containing a wall bonded porous monolithic type polystyrene-divinylbenzene layer in open tubular column format (PLOT). Modification longitudinal homogeneity was monitored using scanning contactless conductivity detection and scanning electron microscopy. The multichannel open tubular capillary column showed channel diameter and polymer layer consistency of 4.2 ± 0.1 μm and 0.26 ± 0.02 μm respectively, and modification of 100% of the parallel channels with the monolithic polymer. The modified multi-channel capillaries were applied to the in-capillary micro-extraction of water samples. 500 μL of water samples containing single μg L−1 levels of polyaromatic hydrocarbons were extracted at a flow rate of 10 μL min−1, and eluted in 50 μL of acetonitrile for analysis using HPLC with fluorescence detection. HPLC LODs were 0.08, 0.02 and 0.05 μg L−1 for acenaphthene, anthracene and pyrene, respectively, with extraction recoveries of between 77 and 103%. The modified capillaries were also investigated briefly for direct application to liquid chromatographic separations, with the retention and elution of a standard protein (cytochrome c) under isocratic conditions demonstrated, proving chromatographic potential of the new column format, with run-to-run retention time reproducibility of below 1%.
Item Details
Item Type: | Refereed Article |
---|---|
Keywords: | photonic crystal fibres; wall modified; micro-extraction; capillary chromatography |
Research Division: | Chemical Sciences |
Research Group: | Analytical chemistry |
Research Field: | Separation science |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the chemical sciences |
UTAS Author: | Kazarian, AA (Dr Tom Kazarian) |
UTAS Author: | Sanz Rodriguez, E (Dr Estrella Sanz Rodriguez) |
UTAS Author: | Deverell, JA (Dr Jeremy Deverell) |
UTAS Author: | Paull, B (Professor Brett Paull) |
ID Code: | 113612 |
Year Published: | 2016 |
Funding Support: | Australian Research Council (DP140104323) |
Web of Science® Times Cited: | 20 |
Deposited By: | Chemistry |
Deposited On: | 2017-01-09 |
Last Modified: | 2017-10-30 |
Downloads: | 0 |
Repository Staff Only: item control page