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Performance of a New 235 nm UV-LED-Based On-Capillary Photometric Detector


Li, Y and Nesterenko, PN and Paull, B and Stanley, R and Macka, M, Performance of a New 235 nm UV-LED-Based On-Capillary Photometric Detector, Analytical Chemistry, 88, (24) pp. 12116-12121. ISSN 0003-2700 (2016) [Refereed Article]

Copyright Statement

Copyright 2016 American Chemical Society

DOI: doi:10.1021/acs.analchem.6b02832


In this work, for the first time, a sub-250 nm light-emitting diode (LED) is investigated as a light source for optical detection in chemical analysis. A 235 nm deep ultraviolet-light-emitting diode (UV-LED) is employed within an on-capillary photometric detector and applied in capillary ion-exchange chromatography (IEC) for the detection of common ultraviolet (UV)-absorbing anions (here, iodide, nitrate, and nitrite). This investigation focused on fundamental properties of UV-LEDs, in particular, emission spectra, radiometric power, effective heat dissipation with a passive heat sink, and energy conversion. The detection showed excellent linearity with stray light down to 0.6%, and an effective path length at 92% of the used capillary inner diameter. The analytical performance parameters were demonstrated by detection of chromatographic separation of iodide in simulated seawater, showing a limit of detection (LOD) of 1.30 μmol L1, a linear range of 7.93937 μmol L1, and reproducibility (with a relative standard deviation (RSD)) of 0.6% for peak height 0.7% for peak area. In addition, nitrite and nitrate were selected to study the potential of using deep UV-LEDs as the light source in photometric detection for even lower-wavelength-absorbing analytes (λmax = 209 nm for nitrite and 200 nm for nitrate), showing reproducibility (RSD = 1.2% and 3.6% for peak height and 0.9% and 2.9% for peak area, respectively) and LOD = 7 and 26 μmol L1.

Item Details

Item Type:Refereed Article
Keywords:LED; Detection; Capillary HPLC
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Li, Y (Mr Yan Li)
UTAS Author:Nesterenko, PN (Professor Pavel Nesterenko)
UTAS Author:Paull, B (Professor Brett Paull)
UTAS Author:Stanley, R (Professor Roger Stanley)
UTAS Author:Macka, M (Professor Mirek Macka)
ID Code:113608
Year Published:2016
Funding Support:Australian Research Council (FT120100559)
Web of Science® Times Cited:40
Deposited By:Chemistry
Deposited On:2017-01-09
Last Modified:2022-08-22

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