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Performance of a New 235 nm UV-LED-Based On-Capillary Photometric Detector
Citation
Li, Y and Nesterenko, PN and Paull, B and Stanley, R and Macka, M, Performance of a New 235 nm UV-LED-Based On-Capillary Photometric Detector, Analytical Chemistry, 88, (24) pp. 12116-12121. ISSN 0003-2700 (2016) [Refereed Article]
Copyright Statement
Copyright 2016 American Chemical Society
DOI: doi:10.1021/acs.analchem.6b02832
Abstract
In this work, for the first time, a sub-250 nm light-emitting diode (LED) is investigated as a light source for optical detection in chemical analysis. A 235 nm deep ultraviolet-light-emitting diode (UV-LED) is employed within an on-capillary photometric detector and applied in capillary ion-exchange chromatography (IEC) for the detection of common ultraviolet (UV)-absorbing anions (here, iodide, nitrate, and nitrite). This investigation focused on fundamental properties of UV-LEDs, in particular, emission spectra, radiometric power, effective heat dissipation with a passive heat sink, and energy conversion. The detection showed excellent linearity with stray light down to 0.6%, and an effective path length at 92% of the used capillary inner diameter. The analytical performance parameters were demonstrated by detection of chromatographic separation of iodide in simulated seawater, showing a limit of detection (LOD) of 1.30 μmol L–1, a linear range of 7.9–3937 μmol L–1, and reproducibility (with a relative standard deviation (RSD)) of 0.6% for peak height 0.7% for peak area. In addition, nitrite and nitrate were selected to study the potential of using deep UV-LEDs as the light source in photometric detection for even lower-wavelength-absorbing analytes (λmax = 209 nm for nitrite and 200 nm for nitrate), showing reproducibility (RSD = 1.2% and 3.6% for peak height and 0.9% and 2.9% for peak area, respectively) and LOD = 7 and 26 μmol L–1.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | LED; Detection; Capillary HPLC |
| Research Division: | Chemical Sciences |
| Research Group: | Analytical chemistry |
| Research Field: | Separation science |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding knowledge |
| Objective Field: | Expanding knowledge in the chemical sciences |
| UTAS Author: | Li, Y (Mr Yan Li) |
| UTAS Author: | Nesterenko, PN (Professor Pavel Nesterenko) |
| UTAS Author: | Paull, B (Professor Brett Paull) |
| UTAS Author: | Stanley, R (Professor Roger Stanley) |
| UTAS Author: | Macka, M (Professor Mirek Macka) |
| ID Code: | 113608 |
| Year Published: | 2016 |
| Funding Support: | Australian Research Council (FT120100559) |
| Web of Science® Times Cited: | 40 |
| Deposited By: | Chemistry |
| Deposited On: | 2017-01-09 |
| Last Modified: | 2017-10-31 |
| Downloads: | 0 |
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