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Laser Doppler Flowmetry to Measure Changes in Cerebral Blood Flow


Sutherland, BA and Rabie, T and Buchan, AM, Laser Doppler Flowmetry to Measure Changes in Cerebral Blood Flow, Cerebral Angiogenesis Methods and Protocols, Humana Press, R Milner (ed), United Kingdom, pp. 237-248. ISBN 978-1-4939-0320-7 (2014) [Research Book Chapter]

Copyright Statement

Copyright 2014 Springer Science+Business Media New York

DOI: doi:10.1007/978-1-4939-0320-7_20


© Springer Science+Business Media New York 2014.Laser Doppler flowmetry (LDF) is a method by which relative cerebral blood flow (CBF) of the cortex can be measured. Although the method is easy to employ, LDF only measures relative CBF, while absolute CBF cannot be quantified. LDF is useful for investigating CBF changes in a number of different applications including neurovascular and stroke research. This chapter will prepare the reader for rodent experiments using LDF with two preparations. The closed skull preparation can be used to monitor CBF with an intact skull, but in adult rats, thinning of the skull is required to obtain an accurate cortical CBF signal. The open skull preparation requires a craniotomy to expose the surface of the brain and the LDF probe is held close to the surface to measure cerebral perfusion.

Item Details

Item Type:Research Book Chapter
Keywords:laser doppler flowmetry, cerebral blood flow, rodent, relative changes, probe, perfusion
Research Division:Biomedical and Clinical Sciences
Research Group:Neurosciences
Research Field:Central nervous system
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the health sciences
UTAS Author:Sutherland, BA (Associate Professor Brad Sutherland)
ID Code:113427
Year Published:2014
Deposited By:Office of the School of Medicine
Deposited On:2016-12-22
Last Modified:2017-11-06

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