eCite Digital Repository

Multi-modal assessment of neurovascular coupling during cerebral ischaemia and reperfusion using remote middle cerebral artery occlusion

Citation

Sutherland, BA and Fordsmann, JC and Martin, C and Neuhaus, AA and Witgen, BM and Piilgaard, H and Lonstrup, M and Couch, Y and Sibson, NR and Lauritzen, M and Buchan, AM, Multi-modal assessment of neurovascular coupling during cerebral ischaemia and reperfusion using remote middle cerebral artery occlusion, Journal of Cerebral Blood Flow and Metabolism, 37, (7) pp. 2494-2508. ISSN 1559-7016 (2017) [Refereed Article]


Preview
PDF
2Mb
  

Copyright Statement

Copyright 2016 the Author(s) Licenced under Creative Commons Attribution 3.0 Unported (CC BY 3.0) https://creativecommons.org/licenses/by/3.0/

DOI: doi:10.1177/0271678X16669512

Abstract

Hyperacute changes in cerebral blood flow during cerebral ischaemia and reperfusion are important determinants of injury. Cerebral blood flow is regulated by neurovascular coupling, and disruption of neurovascular coupling contributes to brain plasticity and repair problems. However, it is unknown how neurovascular coupling is affected hyperacutely during cerebral ischaemia and reperfusion. We have developed a remote middle cerebral artery occlusion model in the rat, which enables multi-modal assessment of neurovascular coupling immediately prior to, during and immediately following reperfusion. Male Wistar rats were subjected to remote middle cerebral artery occlusion, where a long filament was advanced intraluminally through a guide cannula in the common carotid artery. Transcallosal stimulation evoked increases in blood flow, tissue oxygenation and neuronal activity, which were diminished by middle cerebral artery occlusion and partially restored during reperfusion. These evoked responses were not affected by administration of the thrombolytic alteplase at clinically used doses. Evoked cerebral blood flow responses were fully restored at 24 h post–middle cerebral artery occlusion indicating that neurovascular dysfunction was not sustained. These data show for the first time that the rat remote middle cerebral artery occlusion model coupled with transcallosal stimulation provides a novel method for continuous assessment of hyperacute neurovascular coupling changes during ischaemia and reperfusion, and offers unique insight into hyperacute ischaemic pathophysiology.

Item Details

Item Type:Refereed Article
Keywords:neurovascular coupling, stroke, middle cerebral artery occlusion, cerebral blood flow, alteplase
Research Division:Medical and Health Sciences
Research Group:Neurosciences
Research Field:Central Nervous System
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Medical and Health Sciences
Author:Sutherland, BA (Dr Brad Sutherland)
ID Code:113418
Year Published:2017 (online first 2016)
Deposited By:Medicine (Discipline)
Deposited On:2016-12-22
Last Modified:2017-11-08
Downloads:5 View Download Statistics

Repository Staff Only: item control page