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The loss of vernalization requirement in narrow-leafed lupin is associated with a deletion in the promoter and de-repressed expression of a Flowering Locus T (FT) homologue

Citation

Nelson, MN and Ksiazkiewicz, M and Rychel, S and Besharat, N and Taylor, CM and Wyrwa, K and Jost, R and Erskine, W and Cowling, WA and Berger, JD and Batley, J and Weller, JL and Naganowska, B and Wolko, B, The loss of vernalization requirement in narrow-leafed lupin is associated with a deletion in the promoter and de-repressed expression of a Flowering Locus T (FT) homologue, New Phytologist, 213, (1) pp. 220-232. ISSN 1469-8137 (2017) [Refereed Article]

Copyright Statement

Copyright 2016 The Authors

DOI: doi:10.1111/nph.14094

Abstract

Adaptation of Lupinus angustifolius (narrow-leafed lupin) to cropping in southern Australian and northern Europe was transformed by a dominant mutation (Ku) that removed vernalization requirement for flowering. The Ku mutation is now widely used in lupin breeding to confer early flowering and maturity. We report here the identity of the Ku mutation.
  • We used a range of genetic, genomic and gene expression approaches to determine whether Flowering Locus T (FT) homologues are associated with the Ku locus.
  • One of four FT homologues present in the narrow-leafed lupin genome, LanFTc1, perfectly co-segregated with the Ku locus in a reference mapping population. Expression of LanFTc1 in the ku (late-flowering) parent was strongly induced by vernalization, in contrast to the Ku (early-flowering) parent, which showed constitutively high LanFTc1 expression. Co-segregation of this expression phenotype with the LanFTc1 genotype indicated that the Ku mutation impairs cis-regulation of LanFTc1. Sequencing of LanFTc1 revealed a 1.4-kb deletion in the promoter region, which was perfectly predictive of vernalization response in 216 wild and domesticated accessions. Linkage disequilibrium rapidly decayed around LanFTc1, suggesting that this deletion caused the loss of vernalization response.
  • This is the first time a legume FTc subclade gene has been implicated in the vernalization response.
  • Item Details

    Item Type:Refereed Article
    Keywords:flowering time, vernalization, legumes, lupins, domestication
    Research Division:Biological Sciences
    Research Group:Plant Biology
    Research Field:Plant Physiology
    Objective Division:Expanding Knowledge
    Objective Group:Expanding Knowledge
    Objective Field:Expanding Knowledge in the Environmental Sciences
    Author:Weller, JL (Dr Jim Weller)
    ID Code:113160
    Year Published:2017
    Funding Support:Australian Research Council (FT120100048)
    Web of Science® Times Cited:1
    Deposited By:Plant Science
    Deposited On:2016-12-14
    Last Modified:2017-03-17
    Downloads:0

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