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Salinity effects on chloroplast PSII performance in glycophytes and halophytes
Citation
Percey, WJ and McMinn, A and Bose, J and Breadmore, MC and Guijt, RM and Shabala, S, Salinity effects on chloroplast PSII performance in glycophytes and halophytes, Functional Plant Biology, 43, (11) pp. 1003-1015. ISSN 1445-4408 (2016) [Refereed Article]
Copyright Statement
Copyright 2016 CSIRO
DOI: doi:10.1071/FP16135
Abstract
The effects of NaCl stress and K+ nutrition on photosynthetic parameters of isolated chloroplasts were investigated using PAM fluorescence. Intact mesophyll cells were able to maintain optimal photosynthetic performance when exposed to salinity for more than 24 h whereas isolated chloroplasts showed declines in both the relative electron transport rate (rETR) and the maximal photochemical efficiency of PSII (Fv/Fm) within the first hour of treatment. The rETR was much more sensitive to salt stress compared with Fv/Fm, with 40% inhibition of rETR observed at apoplastic NaCl concentration as low as 20 mM. In isolated chloroplasts, absolute K+ concentrations were more essential for the maintenance of the optimal photochemical performance (Fv/Fm values) rather than sodium concentrations per se. Chloroplasts from halophyte species of quinoa (Chenopodium quinoa Willd.) and pigface (Carpobrotus rosii (Haw.) Schwantes) showed less than 18% decline in Fv/Fm under salinity, whereas the Fv/Fm decline in chloroplasts from glycophyte pea (Pisum sativum L.) and bean (Vicia faba L.) species was much stronger (31 and 47% respectively). Vanadate (a P-type ATPase inhibitor) significantly reduced Fv/Fm in both control and salinity treated chloroplasts (by 7 and 25% respectively), whereas no significant effects of gadolinium (blocker of non-selective cation channels) were observed in salt-treated chloroplasts. Tetraethyl ammonium (TEA) (K+ channel inhibitor) and amiloride (inhibitor of the Na+/H+ antiporter) increased the Fv/Fm of salinity treated chloroplasts by 16 and 17% respectively. These results suggest that chloroplasts’ ability to regulate ion transport across the envelope and thylakoid membranes play a critical role in leaf photosynthetic performance under salinity.
Item Details
Item Type: | Refereed Article |
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Keywords: | photosynthesis, potassium, sodium, ROS, membrane transport, non-stomatal limitation |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Environmental Management |
Objective Group: | Terrestrial systems and management |
Objective Field: | Control of pests, diseases and exotic species in terrestrial environments |
UTAS Author: | Percey, WJ (Mr William Percey) |
UTAS Author: | McMinn, A (Professor Andrew McMinn) |
UTAS Author: | Bose, J (Dr Jayakumar Bose) |
UTAS Author: | Breadmore, MC (Professor Michael Breadmore) |
UTAS Author: | Guijt, RM (Dr Rosanne Guijt) |
UTAS Author: | Shabala, S (Professor Sergey Shabala) |
ID Code: | 113147 |
Year Published: | 2016 |
Web of Science® Times Cited: | 23 |
Deposited By: | Ecology and Biodiversity |
Deposited On: | 2016-12-14 |
Last Modified: | 2018-04-19 |
Downloads: | 0 |
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