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Salinity effects on chloroplast PSII performance in glycophytes and halophytes

Citation

Percey, WJ and McMinn, A and Bose, J and Breadmore, MC and Guijt, RM and Shabala, S, Salinity effects on chloroplast PSII performance in glycophytes and halophytes, Functional Plant Biology, 43, (11) pp. 1003-1015. ISSN 1445-4408 (2016) [Refereed Article]

Copyright Statement

Copyright 2016 CSIRO

DOI: doi:10.1071/FP16135

Abstract

The effects of NaCl stress and K+ nutrition on photosynthetic parameters of isolated chloroplasts were investigated using PAM fluorescence. Intact mesophyll cells were able to maintain optimal photosynthetic performance when exposed to salinity for more than 24 h whereas isolated chloroplasts showed declines in both the relative electron transport rate (rETR) and the maximal photochemical efficiency of PSII (Fv/Fm) within the first hour of treatment. The rETR was much more sensitive to salt stress compared with Fv/Fm, with 40% inhibition of rETR observed at apoplastic NaCl concentration as low as 20 mM. In isolated chloroplasts, absolute K+ concentrations were more essential for the maintenance of the optimal photochemical performance (Fv/Fm values) rather than sodium concentrations per se. Chloroplasts from halophyte species of quinoa (Chenopodium quinoa Willd.) and pigface (Carpobrotus rosii (Haw.) Schwantes) showed less than 18% decline in Fv/Fm under salinity, whereas the Fv/Fm decline in chloroplasts from glycophyte pea (Pisum sativum L.) and bean (Vicia faba L.) species was much stronger (31 and 47% respectively). Vanadate (a P-type ATPase inhibitor) significantly reduced Fv/Fm in both control and salinity treated chloroplasts (by 7 and 25% respectively), whereas no significant effects of gadolinium (blocker of non-selective cation channels) were observed in salt-treated chloroplasts. Tetraethyl ammonium (TEA) (K+ channel inhibitor) and amiloride (inhibitor of the Na+/H+ antiporter) increased the Fv/Fm of salinity treated chloroplasts by 16 and 17% respectively. These results suggest that chloroplasts’ ability to regulate ion transport across the envelope and thylakoid membranes play a critical role in leaf photosynthetic performance under salinity.

Item Details

Item Type:Refereed Article
Keywords:photosynthesis, potassium, sodium, ROS, membrane transport, non-stomatal limitation
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant physiology
Objective Division:Environmental Management
Objective Group:Terrestrial systems and management
Objective Field:Control of pests, diseases and exotic species in terrestrial environments
UTAS Author:Percey, WJ (Mr William Percey)
UTAS Author:McMinn, A (Professor Andrew McMinn)
UTAS Author:Bose, J (Dr Jayakumar Bose)
UTAS Author:Breadmore, MC (Professor Michael Breadmore)
UTAS Author:Guijt, RM (Dr Rosanne Guijt)
UTAS Author:Shabala, S (Professor Sergey Shabala)
ID Code:113147
Year Published:2016
Web of Science® Times Cited:23
Deposited By:Ecology and Biodiversity
Deposited On:2016-12-14
Last Modified:2018-04-19
Downloads:0

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