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Comparative performance of four immunological test kits for the detection of paralytic shellfish toxins in Tasmanian shellfish

Citation

Dorantes Aranda, JJ and Campbell, K and Bradbury, A and Elliott, CT and Harwood, DT and Murray, SA and Ugalde, S and Wilson, K and Burgoyne, M and Hallegraeff, GM, Comparative performance of four immunological test kits for the detection of paralytic shellfish toxins in Tasmanian shellfish, Toxicon, 125 pp. 110-119. ISSN 0041-0101 (2017) [Refereed Article]

Copyright Statement

Copyright 2016 Elsevier Ltd.

DOI: doi:10.1016/j.toxicon.2016.11.262

Abstract

Blooms of the toxic dinoflagellate Alexandrium tamarense (Group 1) seriously impacted the Tasmanian shellfish industry during 2012 and 2015, necessitating product recalls and intensive paralytic shellfish toxin (PST) product testing. The performance of four commercial PST test kits, Abraxis™, Europroxima™, Scotia™ and Neogen™, was compared with the official AOAC LC-FLD method for contaminated mussels and oysters. Abraxis and Europroxima kits underestimated PST in 35–100% of samples when using standard protocols but quantification improved when concentrated extracts were further diluted (underestimation ≤18%). The Scotia kit (cut off 0.2–0.7 mg STX-diHCl eq/kg) delivered 0% false negatives, but 27% false positives. Neogen produced 5% false negatives and 13% false positives when the cut off was altered to 0.5–0.6 mg STX-diHCl eq/kg, the introduction of a conversion step eliminated false negatives. Based on their sensitivity, ease of use and performance, the Neogen kit proved the most suitable kit for use with Tasmanian mussels and oysters. Once formally validated for regulatory purposes, the Neogen kit could provide shellfish growers with a rapid tool for harvesting decisions at the farm gate. Effective rapid screening preventing compliant samples undergoing testing using the more expensive and time consuming LC-FLD method will result in significant savings in analytical costs.

Item Details

Item Type:Refereed Article
Keywords:paralytic shellfish poisoning, mussels, oysters, Alexandrium tamarense
Research Division:Technology
Research Group:Agricultural Biotechnology
Research Field:Agricultural Biotechnology Diagnostics (incl. Biosensors)
Objective Division:Health
Objective Group:Public Health (excl. Specific Population Health)
Objective Field:Food Safety
Author:Dorantes Aranda, JJ (Dr Juan Dorantes Aranda)
Author:Ugalde, S (Ms Sarah Ugalde)
Author:Hallegraeff, GM (Professor Gustaaf Hallegraeff)
ID Code:113094
Year Published:2017
Deposited By:Centre for Ecology and Biodiversity
Deposited On:2016-12-12
Last Modified:2017-10-12
Downloads:0

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