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Studies on the differentiation of the human myelomonocytic cell line RC-2A in response to lymphocyte-derived factors

Citation

Lyons, AB and Ashman, LK, Studies on the differentiation of the human myelomonocytic cell line RC-2A in response to lymphocyte-derived factors, Leukemia Research, 11, (9) pp. 797-805. ISSN 0145-2126 (1987) [Refereed Article]

Copyright Statement

Copyright 1987 Pergamon Journals Ltd.

DOI: doi:10.1016/0145-2126(87)90064-6

Abstract

Cells of the human myelomonocytic line RC-2A were induced to differentiate toward macrophages by culturing for up to 12 days in the presence of supernatant from phytohaemagglutinin stimulated human peripheral blood mononuclear cells (PHA-LCM). The process of differentiation was monitored by changes in expression of two-macrophage related enzymes (alpha-naphthol butyrate esterase and acid phosphatase), the changes in expression of the monocyte-macrophage cell surface markers detected by the monoclonal antibodies anti-Mo1 and anti-Mo2, HLA class 2 antigen detected by FMC-14, and alteration in cell morphology. Maturation induced by PHA-LCM was accompanied by a marked decrease in the proliferative potential of the cell population, and a reduced ability to form colonies in semi-solid medium. Induced RC-2A cells were able to stimulate in one-way mixed leukocyte culture more effectively than control cells.

Item Details

Item Type:Refereed Article
Keywords:myelo-monocytic differentiation, differentiation, myeloid cell line, lymphocyte conditioned medium
Research Division:Medical and Health Sciences
Research Group:Cardiorespiratory Medicine and Haematology
Research Field:Haematology
Objective Division:Health
Objective Group:Other Health
Objective Field:Health not elsewhere classified
Author:Lyons, AB (Dr Bruce Lyons)
ID Code:113017
Year Published:1987
Web of Science® Times Cited:3
Deposited By:Medicine (Discipline)
Deposited On:2016-12-07
Last Modified:2017-05-17
Downloads:0

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