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Specific binding of human interleukin-3 and granulocyte-macrophage colony-stimulating factor to human basophils

Citation

Lopez, AF and Lyons, AB and Eglnton, JM and Park, LS and Clark, SC and Vadas, MA, Specific binding of human interleukin-3 and granulocyte-macrophage colony-stimulating factor to human basophils, The Journal of Allergy and Clinical Immunology, 85, (1) pp. 99-102. ISSN 0091-6749 (1990) [Refereed Article]

Copyright Statement

Copyright 1990 Mosby Inc.

DOI: doi:10.1016/0091-6749(90)90229-W

Abstract

The human T cell-derived cytokines interleukin (IL)-3 and granulocyte-macrophage colony-stimulating factor (GM-CSF) were examined for their ability to bind to human basophils. Basophils were obtained from the peripheral blood of a patient with chronic myeloid leukemia undergoing basophilic differentiation after purification on a density gradient of metrizamide. Binding studies with 125I-labeled IL-3 and 125I-labeled GM-CSF demonstrated that basophils express a single class of high-affinity receptors for each of these molecules. Saturation binding curves with 125I-labeled IL-3 revealed that IL-3 bound specifically to basophils, and analysis according to the method of Scatchard revealed that basophils express 800 to 900 receptors per cell with an apparent dissociation constant of 2.6 x 10(-11) mol/L. Saturation-binding curves with 125I-labeled GM-CSF revealed that basophils express 100 to 200 receptors per cell with an apparent dissociation constant of 4 x 10(-11) mol/L. The demonstration of high-affinity receptors for IL-3 and GM-CSF on human basophils suggests a role for these cytokines in the regulation of basophil function.

Item Details

Item Type:Refereed Article
Keywords:cytokine binding basophils
Research Division:Medical and Health Sciences
Research Group:Immunology
Research Field:Cellular Immunology
Objective Division:Health
Objective Group:Other Health
Objective Field:Health not elsewhere classified
Author:Lyons, AB (Dr Bruce Lyons)
ID Code:113013
Year Published:1990
Web of Science® Times Cited:32
Deposited By:Medicine (Discipline)
Deposited On:2016-12-07
Last Modified:2017-03-23
Downloads:0

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