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Simultaneous analysis of immunophenotype and apoptosis of murine thymocytes by single laser flow cytometry
journal contribution
posted on 2023-05-19, 00:08 authored by Alan Lyons, Samuel, K, Sanderson, A, Maddy, AHThe study of the role of apoptosis in thymocyte development has been hampered by the lack of a means of directly immunophenotyping cells undergoing the early phase of apoptosis. This restriction has been overcome by single laser flow cytometry in which apoptosis is detected by Ethidium Bromide (EBr) staining and cell phenotype by binding of FITC-labelled antibody. The initial phase of apoptosis is observed as a cell population that stains faintly with EBr preceding the characteristically bright EBr-staining normally associated with cell death. Here we directly demonstrate using single laser flow cytometry that CD4+ CD8+ CD3low/CD3intermediate thymocytes undergo apoptosis in vitro in response to glucocorticoid treatment.
History
Publication title
Cytometry. Part AVolume
13Issue
8Pagination
809-821ISSN
1552-4922Department/School
Tasmanian School of MedicinePublisher
John Wiley & Sons, Inc.Place of publication
United StatesRights statement
Copyright 1992 Wiley-Liss, Inc.Repository Status
- Restricted