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Simultaneous analysis of immunophenotype and apoptosis of murine thymocytes by single laser flow cytometry
Citation
Lyons, AB and Samuel, K and Sanderson, A and Maddy, AH, Simultaneous analysis of immunophenotype and apoptosis of murine thymocytes by single laser flow cytometry, Cytometry. Part A, 13, (8) pp. 809-821. ISSN 1552-4922 (1992) [Refereed Article]
Copyright Statement
Copyright 1992 Wiley-Liss, Inc.
DOI: doi:10.1002/cyto.990130803
Abstract
The study of the role of apoptosis in thymocyte development has been hampered by the lack of a means of directly immunophenotyping cells undergoing the early phase of apoptosis. This restriction has been overcome by single laser flow cytometry in which apoptosis is detected by Ethidium Bromide (EBr) staining and cell phenotype by binding of FITC-labelled antibody. The initial phase of apoptosis is observed as a cell population that stains faintly with EBr preceding the characteristically bright EBr-staining normally associated with cell death. Here we directly demonstrate using single laser flow cytometry that CD4+ CD8+ CD3low/CD3intermediate thymocytes undergo apoptosis in vitro in response to glucocorticoid treatment.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | programmed cell death, thymocyte deletion, apoptosis, immunophenotype, ethidium bromide |
| Research Division: | Medical and Health Sciences |
| Research Group: | Immunology |
| Research Field: | Cellular Immunology |
| Objective Division: | Health |
| Objective Group: | Other Health |
| Objective Field: | Health not elsewhere classified |
| UTAS Author: | Lyons, AB (Associate Professor Bruce Lyons) |
| ID Code: | 113006 |
| Year Published: | 1992 |
| Web of Science® Times Cited: | 99 |
| Deposited By: | Medicine |
| Deposited On: | 2016-12-07 |
| Last Modified: | 2017-03-30 |
| Downloads: | 0 |
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