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Simultaneous analysis of immunophenotype and apoptosis of murine thymocytes by single laser flow cytometry

Citation

Lyons, AB and Samuel, K and Sanderson, A and Maddy, AH, Simultaneous analysis of immunophenotype and apoptosis of murine thymocytes by single laser flow cytometry, Cytometry. Part A, 13, (8) pp. 809-821. ISSN 1552-4922 (1992) [Refereed Article]

Copyright Statement

Copyright 1992 Wiley-Liss, Inc.

DOI: doi:10.1002/cyto.990130803

Abstract

The study of the role of apoptosis in thymocyte development has been hampered by the lack of a means of directly immunophenotyping cells undergoing the early phase of apoptosis. This restriction has been overcome by single laser flow cytometry in which apoptosis is detected by Ethidium Bromide (EBr) staining and cell phenotype by binding of FITC-labelled antibody. The initial phase of apoptosis is observed as a cell population that stains faintly with EBr preceding the characteristically bright EBr-staining normally associated with cell death. Here we directly demonstrate using single laser flow cytometry that CD4+ CD8+ CD3low/CD3intermediate thymocytes undergo apoptosis in vitro in response to glucocorticoid treatment.

Item Details

Item Type:Refereed Article
Keywords:programmed cell death, thymocyte deletion, apoptosis, immunophenotype, ethidium bromide
Research Division:Medical and Health Sciences
Research Group:Immunology
Research Field:Cellular Immunology
Objective Division:Health
Objective Group:Other Health
Objective Field:Health not elsewhere classified
Author:Lyons, AB (Dr Bruce Lyons)
ID Code:113006
Year Published:1992
Web of Science® Times Cited:99
Deposited By:Medicine (Discipline)
Deposited On:2016-12-07
Last Modified:2017-03-30
Downloads:0

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