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Determination of lymphocyte division by flow cytometry

Citation

Lyons, AB and Parish, CR, Determination of lymphocyte division by flow cytometry, Journal of Immunological Methods, 171, (1) pp. 131-137. ISSN 0022-1759 (1994) [Refereed Article]


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DOI: doi:10.1016/0022-1759(94)90236-4

Abstract

Techniques currently available for determining cell division are able to show one or, at best, a limited number of cell divisions. Other methods exist which can quantify overall division, but tell nothing about the division history of individual cells. Here we present a new technique in which an intracellular fluorescent label is divided equally between daughter cells upon cell division. The technique is applicable to in vitro cell division, as well as in vivo division of adoptively transferred cells, and can resolve multiple successive generations using flow cytometry. The label is fluorescein derived, allowing monoclonal antibodies conjugated to phycoerythrin or other compatible fluorochromes to be used to immunophenotype the dividing cells.

Item Details

Item Type:Refereed Article
Keywords:Lymphocyte division; Flow cytometry; Immunophenotype; 5-(and -6)-Carboxyfluorescein diacetate succinimidyl ester
Research Division:Medical and Health Sciences
Research Group:Immunology
Research Field:Cellular Immunology
Objective Division:Health
Objective Group:Other Health
Objective Field:Health not elsewhere classified
Author:Lyons, AB (Dr Bruce Lyons)
ID Code:113002
Year Published:1994
Web of Science® Times Cited:1281
Deposited By:Medicine (Discipline)
Deposited On:2016-12-07
Last Modified:2017-02-14
Downloads:0

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