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Determination of lymphocyte division by flow cytometry

journal contribution
posted on 2023-05-19, 00:08 authored by Alan Lyons, Parish, CR
Techniques currently available for determining cell division are able to show one or, at best, a limited number of cell divisions. Other methods exist which can quantify overall division, but tell nothing about the division history of individual cells. Here we present a new technique in which an intracellular fluorescent label is divided equally between daughter cells upon cell division. The technique is applicable to in vitro cell division, as well as in vivo division of adoptively transferred cells, and can resolve multiple successive generations using flow cytometry. The label is fluorescein derived, allowing monoclonal antibodies conjugated to phycoerythrin or other compatible fluorochromes to be used to immunophenotype the dividing cells.

History

Publication title

Journal of Immunological Methods

Volume

171

Pagination

131-137

ISSN

0022-1759

Department/School

Tasmanian School of Medicine

Publisher

Elsevier Science Bv

Place of publication

Po Box 211, Amsterdam, Netherlands, 1000 Ae

Rights statement

© 1994 Elsevier Science B.V. All rights reserved

Repository Status

  • Restricted

Socio-economic Objectives

Other health not elsewhere classified

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