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Potential for non-shivering thermogenesis in perfused chicken (Gallus domesticus) muscle

journal contribution
posted on 2023-05-16, 10:45 authored by Eldershaw, TPD, Duchamp, C, Michael ClarkMichael Clark, Colquhoun, EQ
The humoral modulation of resting muscle heat production of chickens (Gallus domesticus) was investigated in vitro. The resting distal lower limb was perfused via the popliteal artery at 25°C without erythrocytes at constant flow. The preparation was stable for at least 3 hr, showing a constant oxygen uptake (MO2) and perfusion pressure as well as adequately maintaining muscle energy charge and creatine phosphate:creatine ratio. Noradrenaline (NOR), adrenaline (ADR) and serotonin (5-HT) each caused a dose-dependent rise in perfusion pressure. NOR and ADR evoked increased MO2 at low doses eventually followed by decreased MO2 at higher agonist concentrations. 5-HT gave smaller but qualitatively similar MO2 effects. The actions of 50 nM NOR were blocked by prazosin (10 μM) and nitroprusside (0.5 mM), but not altered by propranolol (10 μM). NOR-induced stimulatory MO2 changes in the presence of pharmacological concentrations (1 μM) of glucagon were more pronounced and the thermogenic concentration range of NOR was increased. Taken together, these in vitro findings demonstrate a potential for vasoconstrictor-controlled muscle nonshivering thermogenesis in birds as in marsupials and mammals, suggesting that vascular control of muscle MO2 may be a widespread biological mechanism. The possible implications of these findings for avian nonshivering thermogenesis are discussed.

History

Publication title

Comparative Biochemistry and Physiology. A: Comparative Physiology

Volume

117

Issue

4

Pagination

545-554

ISSN

0300-9629

Department/School

Tasmanian School of Medicine

Publisher

Elsevier Science Inc.

Place of publication

United States

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the environmental sciences

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    University Of Tasmania

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