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Shewanella olleyana sp nov., a marine species isolated from a temperate estuary which produces high levels of polyunsaturated fatty acids
journal contribution
posted on 2023-05-18, 23:41 authored by Skerratt, J, John BowmanJohn Bowman, Peter NicholsTwo polyunsaturated fatty acid (PUFA) producing strains (ACEM 6 and ACEM 9T) isolated from a temperate, humic-rich river estuary in Tasmania, Australia, were found to be members of the genus Shewanella. These strains were able to utilize humic compounds (tannic acid) and derivatives (2,6-anthraquinone disulfonate) as sole carbon sources and as electron acceptors for anaerobic respiration. The major fatty acids were typical of the genus Shewanella; however, PUFAs mostly made up of eicosapentaenoic acid were produced at high levels (10.2-23.6% of total fatty acids) and at relatively high incubation temperatures (10.2% at 24 degrees C). Sequence analysis indicated that ACEM 6 and ACEM 9T had identical 16S rDNA sequences and were most closely related to Shewanella japonica (sequence similarity 97.1%). DNA hybridization and phenotypic characteristics confirmed that the isolates constituted a novel species of the genus Shewanella, which is designated Shewanella olleyana sp. nov. (type strain ACEM 9(T) = ACAM 644T = LMG 21437T).
History
Publication title
International Journal of Systematic and Evolutionary MicrobiologyVolume
52Issue
6Pagination
2101-2106ISSN
1466-5026Department/School
Institute for Marine and Antarctic StudiesPublisher
Soc General MicrobiologyPlace of publication
United KingdomRights statement
Copyright 2002 IUMSRepository Status
- Restricted