A novel and effective fibre-based microfluidic methodology was developed to move and isolate charged solutes, biomolecules, and intact bacterial cells, based upon a novel multi-functional 3D printed supporting platform, with potential applications in the fields of microfluidics and biodiagnostics. Various on-fibre electrophoretic techniques are demonstrated to separate, pre-concentrate, move, split, or cut and collect the isolated zones of target solutes, including proteins and live bacterial cells. The use of knotting to link different fibre materials, and the unique ability of this approach to physically concentrate solutes in different locations are shown such that the concentrated solutes can be physically isolated and easily transferred to other fibres. Application of this novel fibre-based technique within a potential diagnostic platform for urinary tract infection is shown, together with the post-electrophoretic incubation of live bacterial cells, demonstrating the cell survival following on-fibre electrophoretic concentration.

Item Type:	Refereed Article
Keywords:	Fibre microfluidics, electrophoresis, bacteria, urinary tract infetion, trap live cells
Research Division:	Chemical Sciences
Research Group:	Analytical chemistry
Research Field:	Bioassays
Objective Division:	Expanding Knowledge
Objective Group:	Expanding knowledge
Objective Field:	Expanding knowledge in the chemical sciences
UTAS Author:	Cabot, JM (Dr Joan Cabot Canyelles)
UTAS Author:	Macdonald, NP (Dr Niall Macdonald)
UTAS Author:	Phung, SC (Ms Sui Phung)
UTAS Author:	Breadmore, MC (Professor Michael Breadmore)
UTAS Author:	Paull, B (Professor Brett Paull)
ID Code:	112411
Year Published:	2016
Web of Science® Times Cited:	15
Deposited By:	Chemistry
Deposited On:	2016-11-09
Last Modified:	2017-10-31
Downloads:	0