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Two-current choice flumes for testing avoidance and preference in aquatic animals


Jutfelt, F and Sundin, J and Raby, GD and Krang, A-S and Clark, TD, Two-current choice flumes for testing avoidance and preference in aquatic animals, Methods in Ecology and Evolution, 8, (3) pp. 379-390. ISSN 2041-210X (2017) [Refereed Article]

Copyright Statement

Copyright 2016 The Authors

DOI: doi:10.1111/2041-210X.12668


  • Aquatic chemical ecology is an important and growing field of research that involves understanding how organisms perceive and respond to chemical cues in their environment. Research assessing the preference or avoidance of a water source containing specific chemical cues has increased in popularity in recent years, and a variety of methods have been described in the scientific literature. Two-current choice flumes have seen the greatest increase in popularity, perhaps because of their potential to address the broadest range of research questions.
  • Here, we review the literature on two-current choice flumes and show that there is a clear absence of standardised methodologies that make comparisons across studies difficult. Some of the main issues include turbulent flows that cause mixing of cues, inappropriate size of choice arenas for the animals, short experiments with stressed animals, failure to report how experiment- and researcher-biases were eliminated, general underreporting of methodological details, underutilisation of collected data, and inappropriate data analyses.
  • In this review we present best-practice guidelines on how to build, test and use two-current choice flumes to measure the behavioural responses of aquatic animals to chemical cues, and provide blueprints for flume construction. The guidelines include steps that can be taken to avoid problems commonly encountered when using two-current choice flumes and analysing the resulting data.
  • This review provides a set of standards that should be followed to ensure data quality, transparency, and replicability in future studies in this field.

Item Details

Item Type:Refereed Article
Keywords:olfactory, chemosensory, behaviour, predator cues, avoidance behaviour, chemical ecology, chemically mediated behaviour, choice tank, ecotoxicology, flume, fluvarium, shuttle box, toxicology, zebrafish
Research Division:Biological Sciences
Research Group:Zoology
Research Field:Animal physiological ecology
Objective Division:Environmental Management
Objective Group:Coastal and estuarine systems and management
Objective Field:Coastal or estuarine biodiversity
UTAS Author:Clark, TD (Dr Timothy Clark)
ID Code:112354
Year Published:2017
Web of Science® Times Cited:48
Deposited By:Ecology and Biodiversity
Deposited On:2016-11-04
Last Modified:2018-08-17

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