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Chromatographic behaviour of synthetic high pressure high temperature diamond in aqueous normal phase chromatography
Citation
Peristyy, A and Paull, B and Nesterenko, PN, Chromatographic behaviour of synthetic high pressure high temperature diamond in aqueous normal phase chromatography, Journal of Chromatography A, 1470 pp. 59-69. ISSN 0021-9673 (2016) [Refereed Article]
Copyright Statement
Copyright 2016 Elsevier B.V.
DOI: doi:10.1016/j.chroma.2016.09.076
Abstract
The chromatographic properties of high pressure high temperature synthesised diamond (HPHT) are investigated under the conditions of hydrophilic interaction liquid chromatography (HILIC). A 50 × 4.6 mm ID stainless steel column packed with HPHT particles of mean diameter 1.6 μm and specific surface area 5.1 m2 g−1 is used. According to the results of acid-base titration with NaOH the purified HPHT batch contains 4.59 μeq g−1 of protogenic, mainly carboxyl- and hydroxyl-, groups, which make this polar adsorbent suitable for use as a stationary phase in HILIC. The retention behaviour of several classes of polar compounds including benzoic and benzenesulfonic acids, nitro- and chlorophenols, various organic bases, and quaternary ammonium compounds are studied using acetonitrile and methanol based mobile phases containing 5–30 v/v% of water. The effects of the buffer pH and concentration, column temperature and organic solvent content on retention of model compounds are also investigated. It is shown that both pH and acetonitrile/methanol ratio in the mobile phase can be used to vary the separation selectivity. Molecular adsorption mechanism (related to aqueous normal phase mode), rather than partitioning is established to be responsible for the retention.
Item Details
Item Type: | Refereed Article |
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Keywords: | hydrophilic interaction liquid chromatography, synthetic diamond, retention mechanism, aqueous normal phase mode |
Research Division: | Chemical Sciences |
Research Group: | Analytical chemistry |
Research Field: | Separation science |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the chemical sciences |
UTAS Author: | Peristyy, A (Dr Anton Peristyy) |
UTAS Author: | Paull, B (Professor Brett Paull) |
UTAS Author: | Nesterenko, PN (Professor Pavel Nesterenko) |
ID Code: | 111991 |
Year Published: | 2016 |
Funding Support: | Australian Research Council (DP150102608) |
Web of Science® Times Cited: | 7 |
Deposited By: | Chemistry |
Deposited On: | 2016-10-19 |
Last Modified: | 2022-08-22 |
Downloads: | 0 |
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