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Chromatographic behaviour of synthetic high pressure high temperature diamond in aqueous normal phase chromatography

Citation

Peristyy, A and Paull, B and Nesterenko, PN, Chromatographic behaviour of synthetic high pressure high temperature diamond in aqueous normal phase chromatography, Journal of Chromatography A, 1470 pp. 59-69. ISSN 0021-9673 (2016) [Refereed Article]

Copyright Statement

Copyright 2016 Elsevier B.V.

DOI: doi:10.1016/j.chroma.2016.09.076

Abstract

The chromatographic properties of high pressure high temperature synthesised diamond (HPHT) are investigated under the conditions of hydrophilic interaction liquid chromatography (HILIC). A 50נ4.6mm ID stainless steel column packed with HPHT particles of mean diameter 1.6μm and specific surface area 5.1m2g−1 is used. According to the results of acid-base titration with NaOH the purified HPHT batch contains 4.59μeqg−1 of protogenic, mainly carboxyl- and hydroxyl-, groups, which make this polar adsorbent suitable for use as a stationary phase in HILIC. The retention behaviour of several classes of polar compounds including benzoic and benzenesulfonic acids, nitro- and chlorophenols, various organic bases, and quaternary ammonium compounds are studied using acetonitrile and methanol based mobile phases containing 530v/v% of water. The effects of the buffer pH and concentration, column temperature and organic solvent content on retention of model compounds are also investigated. It is shown that both pH and acetonitrile/methanol ratio in the mobile phase can be used to vary the separation selectivity. Molecular adsorption mechanism (related to aqueous normal phase mode), rather than partitioning is established to be responsible for the retention.

Item Details

Item Type:Refereed Article
Keywords:hydrophilic interaction liquid chromatography, synthetic diamond, retention mechanism, aqueous normal phase mode
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Peristyy, A (Dr Anton Peristyy)
Author:Paull, B (Professor Brett Paull)
Author:Nesterenko, PN (Professor Pavel Nesterenko)
ID Code:111991
Year Published:2016
Funding Support:Australian Research Council (DP150102608)
Deposited By:Chemistry
Deposited On:2016-10-19
Last Modified:2017-10-30
Downloads:0

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