The chromatographic properties of high pressure high temperature synthesised diamond (HPHT) are investigated under the conditions of hydrophilic interaction liquid chromatography (HILIC). A 50 × 4.6 mm ID stainless steel column packed with HPHT particles of mean diameter 1.6 μm and specific surface area 5.1 m² g⁻¹ is used. According to the results of acid-base titration with NaOH the purified HPHT batch contains 4.59 μeq g⁻¹ of protogenic, mainly carboxyl- and hydroxyl-, groups, which make this polar adsorbent suitable for use as a stationary phase in HILIC. The retention behaviour of several classes of polar compounds including benzoic and benzenesulfonic acids, nitro- and chlorophenols, various organic bases, and quaternary ammonium compounds are studied using acetonitrile and methanol based mobile phases containing 5–30 v/v% of water. The effects of the buffer pH and concentration, column temperature and organic solvent content on retention of model compounds are also investigated. It is shown that both pH and acetonitrile/methanol ratio in the mobile phase can be used to vary the separation selectivity. Molecular adsorption mechanism (related to aqueous normal phase mode), rather than partitioning is established to be responsible for the retention.

Item Type:	Refereed Article
Keywords:	hydrophilic interaction liquid chromatography, synthetic diamond, retention mechanism, aqueous normal phase mode
Research Division:	Chemical Sciences
Research Group:	Analytical chemistry
Research Field:	Separation science
Objective Division:	Expanding Knowledge
Objective Group:	Expanding knowledge
Objective Field:	Expanding knowledge in the chemical sciences
UTAS Author:	Peristyy, A (Dr Anton Peristyy)
UTAS Author:	Paull, B (Professor Brett Paull)
UTAS Author:	Nesterenko, PN (Professor Pavel Nesterenko)
ID Code:	111991
Year Published:	2016
Funding Support:	Australian Research Council (DP150102608)
Web of Science® Times Cited:	7
Deposited By:	Chemistry
Deposited On:	2016-10-19
Last Modified:	2022-08-22
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