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Evaluating Tissue-Specific Recombination in a Pdgfrα-CreERT2 Transgenic Mouse Line

Citation

O'Rourke, M and Cullen, CL and Auderset, L and Pitman, KA and Achatz, D and Gasperini, R and Young, KM, Evaluating Tissue-Specific Recombination in a Pdgfrα-CreERT2 Transgenic Mouse Line, PLoS One, 11, (9) Article e0162858. ISSN 1932-6203 (2016) [Refereed Article]


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Copyright 2016 O'Rourke et al. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.1371/journal.pone.0162858

Abstract

In the central nervous system (CNS) platelet derived growth factor receptor alpha (PDGFRα) is expressed exclusively by oligodendrocyte progenitor cells (OPCs), making the Pdgfrα promoter an ideal tool for directing transgene expression in this cell type. Two Pdgfrα-CreERT2 mouse lines have been generated for this purpose which, when crossed with cre-sensitive reporter mice, allow the temporally restricted labelling of OPCs for lineage-tracing studies. These mice have also been used to achieve the deletion of CNS-specific genes from OPCs. However the ability of Pdgfrα-CreERT2 mice to induce cre-mediated recombination in PDGFRα+ cell populations located outside of the CNS has not been examined. Herein we quantify the proportion of PDGFRα+ cells that become YFP-labelled following Tamoxifen administration to adult Pdgfrα-CreERT2::Rosa26-YFP transgenic mice. We report that the vast majority (>90%) of PDGFRα+ OPCs in the CNS, and a significant proportion of PDGFRα+ stromal cells within the bone marrow (~38%) undergo recombination and become YFP-labelled. However, only a small proportion of the PDGFRα+ cell populations found in the sciatic nerve, adrenal gland, pituitary gland, heart, gastrocnemius muscle, kidney, lung, liver or intestine become YFP-labelled. These data suggest that Pdgfrα-CreERT2 transgenic mice can be used to achieve robust recombination in OPCs, while having a minimal effect on most PDGFRα+ cell populations outside of the CNS.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Neurosciences
Research Field:Cellular Nervous System
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Nervous System and Disorders
Author:O'Rourke, M (Miss Megan O'Rourke)
Author:Cullen, CL (Dr Carlie Cullen)
Author:Auderset, L (Mr Loic Auderset)
Author:Pitman, KA (Ms Kimberley Pitman)
Author:Achatz, D (Ms Daniela Achatz)
Author:Gasperini, R (Dr Rob Gasperini)
Author:Young, KM (Dr Kaylene Young)
ID Code:111525
Year Published:2016
Deposited By:Menzies Institute for Medical Research
Deposited On:2016-09-20
Last Modified:2017-11-06
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