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Screening of paralytic shellfish toxins in freshwater cyanobacteria and chemical confirmation of the toxins in cultured Anabaena circinalis from Australia
Citation
Onodera, H and Oshima, Y and Watanabe, MF and Watanabe, M and Bolch, CJ and Blackburn, S and Yasumoto, T, Screening of paralytic shellfish toxins in freshwater cyanobacteria and chemical confirmation of the toxins in cultured Anabaena circinalis from Australia, Proceedings of the 7th International Conference on Toxic Phytoplankton, 12-16 July 1995, Sendai, Japan, pp. 563-566. (1996) [Refereed Conference Paper]
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Abstract
Screening of paralytic shellfish toxins in
various freshwater cyanobacterial samples was
conducted by a simple systematic method using
HPLC. Several samples gave peaks
indistinguishable from the standard toxins, but all
of them, except for Anabaena circinalis from
Australia, were found to be negative by reinvestigation
of the samples spiked with standards,
with different post-column derivatization
condition or with samples partially purified using
reversed-phase cartridge and charcoal columns.
An Australian isolate of An. circinalis was
cultured on a large scale and its major toxins, C1,
C2, gonyautoxin-2 and 3, were analyzed by mass
spectrometry to determine the definite chemical
confirmation as the toxins of this species. Toxin
productivity of An. circinalis from a large scale
culture was 5 times higher than that of the
dinoflagellate Alexandrium tamarense culture
from Japan.
Item Details
Item Type: | Refereed Conference Paper |
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Keywords: | cyanobacteria, PSP toxins, Australia, Anabaena circinalis |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Phycology (incl. marine grasses) |
Objective Division: | Environmental Management |
Objective Group: | Fresh, ground and surface water systems and management |
Objective Field: | Measurement and assessment of freshwater quality (incl. physical and chemical conditions of water) |
UTAS Author: | Bolch, CJ (Associate Professor Christopher Bolch) |
ID Code: | 111295 |
Year Published: | 1996 |
Deposited By: | Ecology and Biodiversity |
Deposited On: | 2016-09-07 |
Last Modified: | 2016-10-18 |
Downloads: | 0 |
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