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Genetic characterization of strains of Cyanobacteria using PCR-RFLP of the cpcBA intergenic spacer and flanking regions

Citation

Bolch, CJS and Blackburn, SI and Neilan, BA and Grewe, PM, Genetic characterization of strains of Cyanobacteria using PCR-RFLP of the cpcBA intergenic spacer and flanking regions, Journal of Phycology, 32, (3) pp. 445-451. ISSN 0022-3646 (1996) [Refereed Article]

Copyright Statement

Copyright © 1996 Wiley

DOI: doi:10.1111/j.0022-3646.1996.00445.x

Abstract

Oligonucleotide primers, specific for conserved regions of the genes encoding the β- and α-phycocyanin subunits of phycobilisomes (cpcB and cpcA) of cyanobacteria, were used to amplify a DNA fragment containing the intervening intergenic spacer region (cpcBA-IGS) of 19 strains of three morphospecies of cyanobacteria. Six Australian strains were identified as Anabaena circinalis Rabenhorst, six strains were identified as Microcystis aeruginosa Kützing, and seven strains were identified as Nodularia spumigena Mertens. Restriction enzyme digestion of the amplification products from the strains revealed restriction fragment length polymorphism (RFLP) within all three morphospecies. Strains corresponding to M. aeruginosa were highly polymorphic: 11 of the 14 restriction enzymes used displayed RFLPs. The A. circinalis and N. spumigena strains were less variable: three of 14 enzymes and seven of 14 enzymes, respectively, showed RFLPs. The presence of genetic variation between strains within these three divergent morphospecies, which span two orders of cyanobacteria (Chroococcales Wettstein and Nostocales (Borzi) Geitler), show that the cpcBA- IGS fragment has broad application as a molecular marker for intrageneric studies of cyanobacteria systematics and genetics.

Item Details

Item Type:Refereed Article
Keywords:cyanobacteria, DNA, phycocyanin, intergenic spacer
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Phycology (incl. Marine Grasses)
Objective Division:Environment
Objective Group:Environmental and Natural Resource Evaluation
Objective Field:Rural Water Evaluation (incl. Water Quality)
Author:Bolch, CJS (Dr Christopher Bolch)
ID Code:111254
Year Published:1996
Web of Science® Times Cited:58
Deposited By:Centre for Ecology and Biodiversity
Deposited On:2016-09-06
Last Modified:2016-11-24
Downloads:0

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