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Isolation and purification of Australian isolates of the toxic cyanobacterium Microcystis aeruginosa Kutz

Citation

Bolch, CJS and Blackburn, SI, Isolation and purification of Australian isolates of the toxic cyanobacterium Microcystis aeruginosa Kutz, Journal of Applied Phycology, 8, (1) pp. 5-13. ISSN 0921-8971 (1996) [Refereed Article]

Copyright Statement

Copyright 1996 KluwerAcademic Publisher

DOI: doi:10.1007/BF02186215

Abstract

Isolation and laboratory culture ofMicrocystis aeruginosa Kütz. using a growth medium (MLA medium) suitable for both non-axenic and axenic cultures is described. Seventeen established strains ofM. aeruginosa were subjected to one or more of three purification methods: centrifugation cleaning, sulphide gradient selection, and antibiotic treatment (Imipenem®). While each method purified only about half of the strains attempted, the selective application of each method, based on the morphological characteristics of the strains, succeeded in purifying 12 of the 17 strains. Three of the 5 strains not purified were contaminated with a sulphide-tolerant, Imipenem-resistant spirochaete,Spirochaeta cf.aurantia, which could not be detected on normal, broad spectrum bacterial test media. The presence of this bacterial species was detected only by phase contrast and DAPI (4,6-diamidino-2-phenylindole) stained fluorescence microscopy.

Item Details

Item Type:Refereed Article
Keywords:Microcystis aeruginosa, isolation culture methods
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Phycology (incl. Marine Grasses)
Objective Division:Environment
Objective Group:Environmental and Natural Resource Evaluation
Objective Field:Rural Water Evaluation (incl. Water Quality)
Author:Bolch, CJS (Dr Christopher Bolch)
ID Code:111252
Year Published:1996
Web of Science® Times Cited:161
Deposited By:Centre for Ecology and Biodiversity
Deposited On:2016-09-06
Last Modified:2016-11-24
Downloads:0

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