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Isolation and purification of Australian isolates of the toxic cyanobacterium Microcystis aeruginosa Kutz
journal contribution
posted on 2023-05-18, 22:08 authored by Christopher BolchChristopher Bolch, Blackburn, SIIsolation and laboratory culture ofMicrocystis aeruginosa Kütz. using a growth medium (MLA medium) suitable for both non-axenic and axenic cultures is described. Seventeen established strains ofM. aeruginosa were subjected to one or more of three purification methods: centrifugation cleaning, sulphide gradient selection, and antibiotic treatment (Imipenem®). While each method purified only about half of the strains attempted, the selective application of each method, based on the morphological characteristics of the strains, succeeded in purifying 12 of the 17 strains. Three of the 5 strains not purified were contaminated with a sulphide-tolerant, Imipenem-resistant spirochaete,Spirochaeta cf.aurantia, which could not be detected on normal, broad spectrum bacterial test media. The presence of this bacterial species was detected only by phase contrast and DAPI (4,6-diamidino-2-phenylindole) stained fluorescence microscopy.
History
Publication title
Journal of Applied PhycologyVolume
8Pagination
5-13ISSN
0921-8971Department/School
Institute for Marine and Antarctic StudiesPublisher
Kluwer Academic PublPlace of publication
Van Godewijckstraat 30, Dordrecht, Netherlands, 3311 GzRights statement
Copyright 1996 KluwerAcademic PublisherRepository Status
- Restricted