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Preliminary validation of a high docosahexaenoic acid (DHA) and -linolenic acid (ALA) dietary oil blend: tissue fatty acid composition and liver proteome response in Atlantic salmon (Salmo salar) smolts

Citation

Nuez-Ortin, WG and Carter, CG and Wilson, R and Cooke, I and Nichols, PD, Preliminary validation of a high docosahexaenoic acid (DHA) and -linolenic acid (ALA) dietary oil blend: tissue fatty acid composition and liver proteome response in Atlantic salmon (Salmo salar) smolts, PLoS One, 11, (8) Article e0161513. ISSN 1932-6203 (2016) [Refereed Article]


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Copyright 2016 Nuez-Ortin et al. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.1371/journal.pone.0161513

Abstract

Marine oils are important to human nutrition as the major source of docosahexaenoic acid (DHA), a key omega-3 long-chain (≥ C20) polyunsaturated fatty acid (n-3 LC-PUFA) that is low or lacking in terrestrial plant or animal oils. The inclusion of fish oil as main source of n-3 LC-PUFA in aquafeeds is mostly limited by the increasing price and decreasing availability. Fish oil replacement with cheaper terrestrial plant and animal oils has considerably reduced the content of n-3 LC-PUFA in flesh of farmed Atlantic salmon. Novel DHA-enriched oils with high alpha-linolenic acid (ALA) content will be available from transgenic oilseeds plants in the near future as an alternative for dietary fish oil replacement in aquafeeds. As a preliminary validation, we formulated an oil blend (TOFX) with high DHA and ALA content using tuna oil (TO) high in DHA and the flaxseed oil (FX) high in ALA, and assessed its ability to achieve fish oil-like n-3 LC-PUFA tissue composition in Atlantic salmon smolts. We applied proteomics as an exploratory approach to understand the effects of nutritional changes on the fish liver. Comparisons were made between fish fed a fish oil-based diet (FO) and a commercial-like oil blend diet (fish oil + poultry oil, FOPO) over 89 days. Growth and feed efficiency ratio were lower on the TOFX diet. Fish muscle concentration of n-3 LC-PUFA was significantly higher for TOFX than for FOPO fish, but not higher than for FO fish, while retention efficiency of n-3 LC-PUFA was promoted by TOFX relative to FO. Proteomics analysis revealed an oxidative stress response indicative of the main adaptive physiological mechanism in TOFX fish. While specific dietary fatty acid concentrations and balances and antioxidant supplementation may need further attention, the use of an oil with a high content of DHA and ALA can enhance tissue deposition of n-3 LC-PUFA in relation to a commercially used oil blend.

Item Details

Item Type:Refereed Article
Keywords:fish oil replacement, DHA, ALA, proteomics, aquaculture
Research Division:Biological Sciences
Research Group:Biochemistry and Cell Biology
Research Field:Proteomics and Intermolecular Interactions (excl. Medical Proteomics)
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Fisheries - Aquaculture not elsewhere classified
Author:Nuez-Ortin, WG (Mr Waldo Nuez Ortin)
Author:Carter, CG (Professor Chris Carter)
Author:Wilson, R (Dr Richard Wilson)
Author:Nichols, PD (Dr Peter Nichols)
ID Code:111025
Year Published:2016
Deposited By:Central Science Laboratory
Deposited On:2016-08-26
Last Modified:2017-10-18
Downloads:42 View Download Statistics

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