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Apoptotic Effects of celecoxib derivatives on MCF-7 and K562 cell lines
Citation
Norouzi, S, Apoptotic Effects of celecoxib derivatives on MCF-7 and K562 cell lines (2014) [Masters Research]
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Abstract
Apoptosis is an intricate molecular program with favorable application in many tumor
treatments. Much effort has been directed towards the search for compounds that
influence apoptosis and understanding their mechanisms of action. Cyclooxygenase (COX)-2
inhibitors may induce apoptosis through the COX-2-independent mechanism. In view of the
reported anti proliferative activities of compounds A (3-(4-chlorophenyl) -5-(4-flurophenyl)-
4-phenyl-4, 5-dihydro-1,2,4-oxadiazole) and B (4-Phenyl-3,5-bis(4-chlorophenyl)-4,5-
dihydro-1,2,4-oxadiazole) as two COX-2 inhibitor derivatives in leukemia (K562) and breast
cancer cells (MCF-7), the present study was undertaken to evaluate the potential of these
compounds to induce apoptosis and unravel the associated mechanisms. Compounds A and
B-treated K562 and MCF-7 cells revealed the apoptotic cell death, as confirmed by the
increased annexin-V/PI staining. Further investigation showed that compounds A and B
inhibited nuclear factor kappa-light-chain-enhancer of activated B cells (NF-ĸB), ferritin
heavy chain (FHC) and extra cellular signal-regulated kinase (ERK) activation, whereas it
could not change c-Myc and early growth response protein 1(EGR-1) expression
dramatically. Altogether, our data suggest that induction of apoptosis by compounds A and
B is not associated with COX-2 expression and occurs through the NF-ĸB pathway, which
sequentially inhibits P-ERK and FHC expression.
Item Details
Item Type: | Masters Research |
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Keywords: | Celecoxib derivatives, Leukaemia, breast cancer, apoptosis |
Research Division: | Biological Sciences |
Research Group: | Biochemistry and cell biology |
Research Field: | Signal transduction |
Objective Division: | Manufacturing |
Objective Group: | Human pharmaceutical products |
Objective Field: | Human pharmaceutical treatments |
UTAS Author: | Norouzi, S (Mrs Shaghayegh Norouzi) |
ID Code: | 111007 |
Year Published: | 2014 |
Deposited By: | Health Sciences |
Deposited On: | 2016-08-25 |
Last Modified: | 2016-08-26 |
Downloads: | 0 |
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