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Automated non-invasive video-microscopy of oyster spat heart rate during acute temperature change: impact of acclimation temperature

Citation

Domnik, NJ and Polymeropoulos, ET and Elliott, NG and Frappell, PB and Fisher, JT, Automated non-invasive video-microscopy of oyster spat heart rate during acute temperature change: impact of acclimation temperature, Frontiers in Physiology, 7 Article 236. ISSN 1664-042X (2016) [Refereed Article]


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Copyright 2016 The Authors Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.3389/fphys.2016.00236

Abstract

We developed an automated, non-invasive method to detect real-time cardiac contraction in post-larval (1.11.7 mm length), juvenile oysters (i.e., oyster spat) via a fiber-optic trans-illumination system. The system is housed within a temperature-controlled chamber and video microscopy imaging of the heart was coupled with video edge-detection to measure cardiac contraction, inter-beat interval, and heart rate (HR). We used the method to address the hypothesis that cool acclimation (10C vs. 22CTa10 or Ta22, respectively; each n = 8) would preserve cardiac phenotype (assessed via HR variability, HRV analysis and maintained cardiac activity) during acute temperature changes. The temperature ramp (TR) protocol comprised 2C steps (10 min/experimental temperature, Texp) from 22C to 10C to 22C. HR was related to Texp in both acclimation groups. Spat became asystolic at low temperatures, particularly Ta22 spat (Ta22: 8/8 vs. Ta10: 3/8 asystolic at Texp = 10C). The rate of HR decrease during cooling was less in Ta10 vs. Ta22 spat when asystole was included in analysis (P = 0.026). Time-domain HRV was inversely related to temperature and elevated in Ta10 vs. Ta22 spat (P < 0.001), whereas a lack of defined peaks in spectral density precluded frequency-domain analysis. Application of the method during an acute cooling challenge revealed that cool temperature acclimation preserved active cardiac contraction in oyster spat and increased time-domain HRV responses, whereas warm acclimation enhanced asystole. These physiologic changes highlight the need for studies of mechanisms, and have translational potential for oyster aquaculture practices.

Item Details

Item Type:Refereed Article
Keywords:oyster, oyster spat, video microscopy, acclimation temperature, heart rate, heart rate variability, aquaculture
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Aquaculture Oysters
Author:Polymeropoulos, ET (Dr Elias Polymeropoulos)
Author:Elliott, NG (Dr Nick Elliott)
Author:Frappell, PB (Professor Peter Frappell)
ID Code:110989
Year Published:2016
Web of Science® Times Cited:1
Deposited By:Sustainable Marine Research Collaboration
Deposited On:2016-08-25
Last Modified:2018-03-15
Downloads:65 View Download Statistics

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