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Enriched retinal ganglion cells derived from human embryonic stem cells

Citation

Gill, KP and Hung, SSC and Sharov, A and Lo, CY and Needham, K and Lidgerwood, GE and Jackson, S and Crombie, DE and Nayagam, BA and Cook, AL and Hewitt, AW and Pebay, A and Wong, RCB, Enriched retinal ganglion cells derived from human embryonic stem cells, Scientific Reports, 6 Article 30552. ISSN 2045-2322 (2016) [Refereed Article]


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Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.1038/srep30552

Abstract

Optic neuropathies are characterised by a loss of retinal ganglion cells (RGCs) that lead to vision impairment. Development of cell therapy requires a better understanding of the signals that direct stem cells into RGCs. Human embryonic stem cells (hESCs) represent an unlimited cellular source for generation of human RGCs in vitro. In this study, we present a 45-day protocol that utilises magnetic activated cell sorting to generate enriched population of RGCs via stepwise retinal differentiation using hESCs. We performed an extensive characterization of these stem cell-derived RGCs by examining the gene and protein expressions of a panel of neural/RGC markers. Furthermore, whole transcriptome analysis demonstrated similarity of the hESC-derived RGCs to human adult RGCs. The enriched hESC-RGCs possess long axons, functional electrophysiological profiles and axonal transport of mitochondria, suggestive of maturity. In summary, this RGC differentiation protocol can generate an enriched population of functional RGCs from hESCs, allowing future studies on disease modeling of optic neuropathies and development of cell therapies.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Ophthalmology and Optometry
Research Field:Ophthalmology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Hearing, Vision, Speech and Their Disorders
Author:Cook, AL (Dr Tony Cook)
Author:Hewitt, AW (Professor Alex Hewitt)
ID Code:110837
Year Published:2016
Web of Science® Times Cited:13
Deposited By:Menzies Institute for Medical Research
Deposited On:2016-08-17
Last Modified:2017-11-06
Downloads:102 View Download Statistics

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