eCite Digital Repository

Modular development of a prototype point of care molecular diagnostic platform for sexually transmitted infections

Citation

Branavan, M and Mackay, RE and Craw, P and Naveenathayalan, A and Ahern, JC and Sivanesan, T and Hudson, C and Stead, T and Kremer, J and Garg, N and Baker, M and Sadiq, ST and Balachandran, W, Modular development of a prototype point of care molecular diagnostic platform for sexually transmitted infections, Medical Engineering and Physics, 38, (8) pp. 741-748. ISSN 1350-4533 (2016) [Refereed Article]


Preview
PDF
2Mb
  

Copyright Statement

Copyright 2016 The Author(s). Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.1016/j.medengphy.2016.04.022

Abstract

This paper presents the design of a modular point of care test platform that integrates a proprietary sample collection device directly with a microfluidic cartridge. Cell lysis, within the cartridge, is conducted using a chemical method and nucleic acid purification is done on an activated cellulose membrane. The microfluidic device incorporates passive mixing of the lysis-binding buffers and sample using a serpentine channel. Results have shown extraction efficiencies for this new membrane of 69% and 57% compared to the commercial Qiagen extraction method of 85% and 59.4% for 0.1áng/ÁL and 100áng/ÁL salmon sperm DNA respectively spiked in phosphate buffered solution. Extraction experiments using the serpentine passive mixer cartridges incorporating lysis and nucleic acid purification showed extraction efficiency around 80% of the commercial Qiagen kit. Isothermal amplification was conducted using thermophillic helicase dependant amplification and recombinase polymerase amplification. A low cost benchtop real-time isothermal amplification platform has been developed capable of running six amplifications simultaneously. Results show that the platform is capable of detecting 1.32Î106 of sample DNA through thermophillic helicase dependant amplification and 1Î105 copy numbers Chlamydia trachomatis genomic DNA within 10ámin through recombinase polymerase nucleic acid amplification tests.

Item Details

Item Type:Refereed Article
Keywords:microfluidics, microengineering, point-of-care, molecular diagnostics
Research Division:Chemical Sciences
Research Group:Medicinal and Biomolecular Chemistry
Research Field:Molecular Medicine
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Medical and Health Sciences
Author:Craw, P (Dr Pascal Craw)
ID Code:110687
Year Published:2016
Web of Science® Times Cited:7
Deposited By:Chemistry
Deposited On:2016-08-09
Last Modified:2017-02-27
Downloads:45 View Download Statistics

Repository Staff Only: item control page