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Three-step stacking by field-enhanced sample injection, sweeping, and micelle to solvent stacking in capillary electrophoresis: Anionic analytes

Citation

Grochocki, W and Markuszewski, MJ and Quirino, JP, Three-step stacking by field-enhanced sample injection, sweeping, and micelle to solvent stacking in capillary electrophoresis: Anionic analytes, Journal of Chromatography A, 1442 pp. 140-143. ISSN 0021-9673 (2016) [Refereed Article]

Copyright Statement

Copyright 2016 Elsevier B.V.

DOI: doi:10.1016/j.chroma.2016.03.002

Abstract

Three-step stacking by field-enhanced sample injection (FESI), sweeping, and micelle to solvent stacking (MSS) in co-EOF capillary zone electrophoresis (CZE) is presented for anionic analytes. Long FESI produced an overloaded stacked zone of analytes (four model penicillins). Sweeping of the FESI zone was by electrokinetic injection of cetyltrimethylammonium bromide (CTAB) micelles. MSS was by short injection of 60% methanol that released the swept analytes from CTAB micelles. The sensitivity enhancement factors were 146–279 and 519–954 for conductivity ratio of 10 and 100, respectively. The SEF enhancement factors (factor = SEF from three-step stacking/SEF from FESI) were 16–32 and 6–10, correspondingly. The LODs were between 6.6–13.2 ng/mL, repeatability (intraday and interday) was %RSD ≤ 5.4%, and linearity was R2 ≥ 0.998. Application to real sample was investigated using fortified plasma after liquid–liquid extraction.

Item Details

Item Type:Refereed Article
Keywords:stacking, capillary electrophoresis, field-enhanced sample injection, sweeping, micelle to solvent stacking, anionic analytes
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Flow Analysis
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Grochocki, W (Mr Wojciech Grochocki)
Author:Quirino, JP (Associate Professor Lito Quirino)
ID Code:110672
Year Published:2016
Deposited By:Chemistry
Deposited On:2016-08-09
Last Modified:2017-11-01
Downloads:0

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