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High-performance liquid chromatographic assay for the simultaneous determination of ipratropium bromide, fenoterol, salbutamol and terbutaline in nebulizer solution

Citation

Jacobson, GA and Peterson, GM, High-performance liquid chromatographic assay for the simultaneous determination of ipratropium bromide, fenoterol, salbutamol and terbutaline in nebulizer solution, Journal of Pharmaceutical and Biomedical Analysis, 12, (6) pp. 825-32. ISSN 0731-7085 (1994) [Refereed Article]

DOI: doi:10.1016/0731-7085(94)E0006-M

Abstract

A reversed-phase ion-pair high-performance liquid chromatography assay was developed for the simultaneous determination of ipratropium bromide, fenoterol hydrobromide, salbutamol sulphate and terbutaline sulphate in nebulizer solution. Chromatographic separation was achieved with a Nova-Pak C18 4 microns 10 cm x 8 mm i.d. Radial-pak cartridge inside a Waters RCM 8 x 10 compression module using ternary gradient analysis. Detection was performed using UV detection at 220 nm. The standard curves were linear over the following ranges: ipratropium bromide 20.8-250.0 micrograms ml-1, fenoterol hydrobromide 27.8-500.0 micrograms ml-1, salbutamol sulphate 34.7-2500.0 micrograms ml-1 and terbutaline sulphate 69.5-2500 micrograms ml-1. Inter-day and intra-day relative standard deviations for each compound ranged from 4.5-5.2% and 3.5-3.9%, respectively. The assay procedure was developed to allow the accurate determination of constituents in various combinations of nebulizer solution, as well as for stability indicating purposes. This provides a convenient means of testing long-term compatibility and stability following the post-manufacture mixing of commonly used nebulized preparations.

Item Details

Item Type:Refereed Article
Keywords:salbutamol assay nebuliser
Research Division:Medical and Health Sciences
Research Group:Pharmacology and Pharmaceutical Sciences
Research Field:Pharmaceutical Sciences
Objective Division:Health
Objective Group:Other Health
Objective Field:Health not elsewhere classified
UTAS Author:Jacobson, GA (Associate Professor Glenn Jacobson)
UTAS Author:Peterson, GM (Professor Gregory Peterson)
ID Code:110296
Year Published:1994
Web of Science® Times Cited:36
Deposited By:Pharmacy
Deposited On:2016-07-22
Last Modified:2016-07-25
Downloads:0

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