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Effects of anaesthetics containing eugenol on Neoparamoeba perurans


Shijie, L and Adams, MB and Nowak, BF and Crosbie, PBB, Effects of anaesthetics containing eugenol on Neoparamoeba perurans, Aquaculture, 463 pp. 159-162. ISSN 0044-8486 (2016) [Refereed Article]

Copyright Statement

ę 2016 Published by Elsevier B.V.

DOI: doi:10.1016/j.aquaculture.2016.05.033


Oil extracted from cloves, Syzygium aromaticum, consists mainly of eugenol, which has been documented to affect protozoa attachment, viability and growth. Isoeugenol (sold under the trade name AQUI-S«) is a commercially available fish anaesthetic produced by isomerisation of eugenol. Both are anaesthetics commonly employed during the husbandry and euthanasia of Atlantic salmon, Salmo salar L., used for amoebic gill disease (AGD) research. Neoparamoeba perurans (the causative agent of AGD) trophozoites were exposed to clove oil at 10, 20, 40 and 80áμLáL−1 for 10ámin and 5, 10, 20 and 40áμLáL−1 for 120ámin; AQUI-S« at 5, 10, 20 and 40áμLáL−1 for 10ámin and 2.5, 5, 10 and 20áμLáL−1 for 120ámin There were no significant differences in viability and survival of trophozoites after exposure to the anaesthetics. When trophozoites were exposed to clove oil at 80áμLáL−1 for 10ámin there was significantly (Pá<á0.05) more detachment from a plastic surface compared to the control. Continued use of clove oil and AQUI-S« at 40áμLáL−1 or less for up to 120ámin is unlikely to have a detrimental impact on amoebae that are isolated and collected from salmon with AGD to be used for downstream research such as projects involving disease challenges and in vitro screening of anti-N. perurans compounds.

Item Details

Item Type:Refereed Article
Keywords:Atlantic salmon, Neoparamoeba perurans, clove oil, anaesthetics, amoebic gill disease, AQUI-S
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Fisheries sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - aquaculture
Objective Field:Fisheries - aquaculture not elsewhere classified
UTAS Author:Shijie, L (Mr Paul Li)
UTAS Author:Adams, MB (Dr Mark Adams)
UTAS Author:Nowak, BF (Professor Barbara Nowak)
UTAS Author:Crosbie, PBB (Dr Philip Crosbie)
ID Code:110234
Year Published:2016
Web of Science® Times Cited:5
Deposited By:Fisheries and Aquaculture
Deposited On:2016-07-21
Last Modified:2017-11-03

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