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A multiplexed set of microsatellite markers for discriminating Acacia mangium, A. auriculiformis, and their hybrid


Le, S and Ratnam, W and Harwood, CE and Larcombe, MJ and Griffin, RA and Koutoulis, A and Harbard, JL and Cyer, KS and Yee, LW and Ha, TH and Vaillancourt, RE, A multiplexed set of microsatellite markers for discriminating Acacia mangium, A. auriculiformis, and their hybrid, Tree Genetics and Genomes, 12, (2) Article 31. ISSN 1614-2942 (2016) [Refereed Article]

Copyright Statement

Copyright 2016 Springer Verlag

DOI: doi:10.1007/s11295-016-0990-2


In order to assist breeding and gene pool conservation in tropical Acacias, we aimed to develop a set of multipurpose SSR markers for use in both Acacia mangium and A. auriculiformis. A total of 51 SSR markers (developed in A. mangium and natural A. mangium x A. auriculiformis hybrid) were tested. A final set of 16 well-performing SSR markers were identified, six of which were species diagnostic. The markers were optimized for assay in four multiplex mixes and used to genotype range-wide samples of A. mangium, A. auriculiformis, and putative F1 hybrids. Simulation analysis was used to investigate the power of the markers for identifying the pure species and their F1, F2, and backcross hybrids. The six species diagnostic markers were particularly powerful for detecting F1 hybrids from pure species but could also discriminate the pure species from F2 and backcross progenies in most cases (97%). STRUCTURE analysis using all 16 markers was likewise able to distinguish these cross types and pure species sets. Both sets of markers had difficulties in distinguishing F2 and backcross progenies. However, identifying F1 from pure species is the current primary concern in countries where these species are planted. The SSR marker set also has direct application in DNA profiling (probability of identity = 4.1  10−13), breeding system analysis, and population genetics.

Item Details

Item Type:Refereed Article
Keywords:Acacia, population genetics, breeding population, hybrid discrimination, microsatellite
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Forestry sciences
Research Field:Tree improvement (incl. selection and breeding)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Forestry
Objective Field:Hardwood plantations
UTAS Author:Le, S (Mr Son Le)
UTAS Author:Harwood, CE (Mr Christopher Harwood)
UTAS Author:Larcombe, MJ (Mr Matthew Larcombe)
UTAS Author:Griffin, RA (Professor Rod Griffin)
UTAS Author:Koutoulis, A (Professor Anthony Koutoulis)
UTAS Author:Harbard, JL (Ms Jane Harbard)
UTAS Author:Vaillancourt, RE (Professor Rene Vaillancourt)
ID Code:110056
Year Published:2016
Web of Science® Times Cited:15
Deposited By:Plant Science
Deposited On:2016-07-12
Last Modified:2018-03-16

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