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Broad spectral excitation of opsin for enhanced stimulation of cells

Citation

Satpathy, S and Batabyal, S and Dhakal, KR and Lin, J and Kim, Y-T and Mohanty, SK, Broad spectral excitation of opsin for enhanced stimulation of cells, Optics Letters, 40, (11) pp. 2465-2468. ISSN 0146-9592 (2015) [Refereed Article]

Copyright Statement

2015 Optical Society of America

DOI: doi:10.1364/OL.40.002465

Abstract

Optical stimulation of cells expressing light-sensitive proteins (opsins) has allowed targeted activation with cellular specificity. However, since narrow-band light has been used for excitation of these optogenetic probes, only active stimulation strategies are being attempted for clinical applications such as restoration of vision. Here, we report use of broad spectral excitation (white light) for optogenetic stimulation of opsin-sensitized cells. We found that ReaChR is optimally excited with white light offering significantly higher photocurrents compared to spectrally filtered narrow-band light stimulation. Our findings open up the possibility of passive stimulation strategy by use of natural sunlight for retinal stimulation, which could have benefits for ambient light stimulated vision restoration.

Item Details

Item Type:Refereed Article
Keywords:medical optics and biotechnology, microscopy, medical optics instrumentation
Research Division:Medical and Health Sciences
Research Group:Other Medical and Health Sciences
Research Field:Medical and Health Sciences not elsewhere classified
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Medical and Health Sciences
Author:Lin, J (Dr John Lin)
ID Code:110054
Year Published:2015
Web of Science® Times Cited:2
Deposited By:Medicine (Discipline)
Deposited On:2016-07-12
Last Modified:2016-11-16
Downloads:0

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