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Broad spectral excitation of opsin for enhanced stimulation of cells
journal contribution
posted on 2023-05-18, 20:45 authored by Satpathy, S, Batabyal, S, Dhakal, KR, John LinJohn Lin, Kim, Y-T, Mohanty, SKOptical stimulation of cells expressing light-sensitive proteins (opsins) has allowed targeted activation with cellular specificity. However, since narrow-band light has been used for excitation of these optogenetic probes, only active stimulation strategies are being attempted for clinical applications such as restoration of vision. Here, we report use of broad spectral excitation (white light) for optogenetic stimulation of opsin-sensitized cells. We found that ReaChR is optimally excited with white light offering significantly higher photocurrents compared to spectrally filtered narrow-band light stimulation. Our findings open up the possibility of passive stimulation strategy by use of natural sunlight for retinal stimulation, which could have benefits for ambient light stimulated vision restoration.
History
Publication title
Optics LettersVolume
40Issue
11Pagination
2465-2468ISSN
0146-9592Department/School
Tasmanian School of MedicinePublisher
Optical Society of AmericaPlace of publication
United StatesRights statement
© 2015 Optical Society of AmericaRepository Status
- Restricted