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Mating-type gene structure and spatial distribution of Didymella tanaceti in pyrethrum fields


Pearce, T and Scott, JB and Hay, FS and Pethybridge, SJ, Mating-type gene structure and spatial distribution of Didymella tanaceti in pyrethrum fields, Phytopathology, 106, (12) pp. 1521-1529. ISSN 1943-7684 (2016) [Refereed Article]

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Copyright 2016 The American Phytopathological Society

DOI: doi:10.1094/PHYTO-01-16-0038-R


Tan spot of pyrethrum (Tanacetum cinerariifolium) is caused by the ascomycete, Didymella tanaceti. To assess the evolutionary role of ascospores in the assumed asexual species, the structure and arrangement of mating-type (MAT) genes were examined. A single MAT1-1 or MAT1-2 idiomorph was identified in all isolates examined, indicating the species is heterothallic. The idiomorphs were flanked upstream and downstream by regions encoding pyridoxamine phosphate oxidase-like and DNA lyase-like proteins, respectively. A multiplex MAT-specific PCR assay was developed and used to genotype 325 isolates collected within two transects in each of four fields in Tasmania, Australia. The ratio of mating-types in each transect were consistent with a 1:1 ratio. The spatial distribution of the mating-types within each transect was random, for all except one transect for MAT1-1 isolates, indicating that clonal patterns of each mating-type were absent. However, evidence of a reduced selection pressure on MAT1-1 isolates was observed, with a second haplotype of the MAT1-1-1 gene identified in 4.4% of MAT1-1 isolates. In vitro crosses between isolates with opposite mating-types failed to produce ascospores. While the sexual morph could not be induced, the occurrence of both mating-types in equal frequencies suggested a cryptic sexual mode of reproduction may occur within field populations.

Item Details

Item Type:Refereed Article
Keywords:tan spot, ascospores, population biology, sexual recombination, genetics, mycology
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant pathology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Horticultural crops
Objective Field:Horticultural crops not elsewhere classified
UTAS Author:Pearce, T (Dr Tamieka Pearce)
UTAS Author:Scott, JB (Dr Jason Scott)
UTAS Author:Pethybridge, SJ (Dr Sarah Pethybridge)
ID Code:110040
Year Published:2016
Web of Science® Times Cited:7
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2016-07-12
Last Modified:2017-11-06

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