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Potato root exudates stimulate zoospore release of Spongospora subterranea


Balendres, M and Nichols, D and Tegg, R and Wilson, C, Potato root exudates stimulate zoospore release of Spongospora subterranea, 8th Australasian Soilborne Diseases Symposium 2014, 10-13 November 2014, Hobart, Australia (2014) [Conference Extract]


Spongospora subterranea is a soil-borne pathogen which causes powdery scab and root infection in potato. Root infection is less well studied than powdery scab, but is now gaining more attention. The role of root exudates in zoospore release of S. subterranea hasn’t been fully investigated and the metabolic profile of potato root exudates, which may influence zoospore releases, is also unreported. We studied the effect of root exudates, from three-week old potato cv. Gladiator (resistant) and cv. Agria (susceptible), on S. subterranea zoospore release in two laboratory assays. Sporosori (2.3 x 104) were added to root exudates and distilled water (control), incubated at fluctuating temperature of 15-18 °C for 10 days, and zoospores were assessed microscopically. We also report the qualitative metabolic profile of root exudates characterised by ultra performance liquid chromatography – mass spectrometry (UPLC-MS). Release of zoospores was observed as early as four days after incubation (DAI) in all test exudates and none in the control solutions. Significant differences were observed between root exudates and distilled water at 4, 7 and 10 DAI in both experiments (P<0.01). Qualitative UPLC-MS chromatogram characterisation of root exudates detected 43 low molecular weight organic compounds, out of 135 analytes screened. This study provided strong evidence root exudates released by potato as early as three weeks from planting stimulates S. subterranea zoospore release. Moreover, potential root metabolites responsible for the observed effect were successfully determined by single UPLC-MS method.

Item Details

Item Type:Conference Extract
Keywords:Spongospora subterranea, host-pathogen interaction, potato, root exudates, zoospores
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Horticultural production
Research Field:Horticultural crop protection (incl. pests, diseases and weeds)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Horticultural crops
Objective Field:Field grown vegetable crops
UTAS Author:Balendres, M (Mr Mark Balendres)
UTAS Author:Nichols, D (Dr David Nichols)
UTAS Author:Tegg, R (Dr Robert Tegg)
UTAS Author:Wilson, C (Professor Calum Wilson)
ID Code:109580
Year Published:2014
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2016-06-22
Last Modified:2016-06-24

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