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The single evolutionary origin of chlorinated auxin provides a phylogenetically informative trait in the Fabaceae


Lam, HK and Ross, JJ and McAdam, EL and McAdam, SAM, The single evolutionary origin of chlorinated auxin provides a phylogenetically informative trait in the Fabaceae, Plant Signaling and Behavior, 11, (7) Article e1197467. ISSN 1559-2316 (2016) [Refereed Article]

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Copyright 2016 Taylor & Francis Group, LLC

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DOI: doi:10.1080/15592324.2016.1197467


Chlorinated auxin (4-chloroindole-3-acetic acid, 4-Cl-IAA), a highly potent plant hormone, was once thought to be restricted to species of the tribe Fabeae within the Fabaceae, until we recently detected this hormone in the seeds of Medicago, Melilotus and Trifolium species. The absence of 4-Cl-IAA in the seeds of the cultivated species Cicer aeritinum from the Cicerae tribe, immediately basal to the Fabeae and Trifolieae tribes, suggested a single evolutionary origin of 4-Cl-IAA. Here, we provide a more robust phylogenetic placement of the ability to produce chlorinated auxin by screening key species spanning this evolutionary transition. We report no detectable level of 4-Cl-IAA in Cicer echinospermum (a wild relative of C. aeritinum) and 4 species (Galega officinalis, Parochetus communis, Astragalus propinquus and A. sinicus) from tribes or clades more basal or sister to the Cicerae tribe. We did detect 4-Cl-IAA in the dry seeds of 4 species from the genus Ononis that are either basal to the genera Medicago, Melilotus and Trigonella or basal to, but still within, the Fabeae and Trifolieae (ex. Parochetus) clades. We conclude that the single evolutionary origin of this hormone in seeds can be used as a phylogenetically informative trait within the Fabaceae.

Item Details

Item Type:Refereed Article
Keywords:chlorinated auxin (4-Cl-IAA), seeds, Fabaceae, Fabeae, Trifolieae, Cicerae, phylogeny, evolution, Ononis, auxin
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Lam, HK (Mr Alvin Lam)
UTAS Author:Ross, JJ (Associate Professor John Ross)
UTAS Author:McAdam, EL (Dr Erin McAdam)
UTAS Author:McAdam, SAM (Dr Scott McAdam)
ID Code:109420
Year Published:2016
Funding Support:Australian Research Council (DE140100946)
Web of Science® Times Cited:1
Deposited By:Plant Science
Deposited On:2016-06-15
Last Modified:2017-11-06

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