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Direct electrokinetic injection of inorganic cations from whole fruits and vegetables for capillary electrophoresis analysis

journal contribution
posted on 2023-05-18, 19:27 authored by Umme Kalsoom, Rosanne Guijt, Boyce, MC, Ashley TownsendAshley Townsend, Haselberg, R, Michael BreadmoreMichael Breadmore
A novel approach for the direct injection from plant tissues without any sample pre-treatment has been developed by simply placing a small piece of the tissue into a capillary electrophoresis vial followed by application of a voltage for electrokinetic injection. Separations of sodium, potassium, calcium and magnesium were achieved using a BGE comprising 10 mM imidazole and 2.5 mM 18-crown-6-ether at pH 4.5. The addition of 2% (m/v) hydroxypropylmethyl cellulose to the separation buffer allowed for precise and accurate electrokinetic injection of ions from the plant material by halting the movement of tissue fluid into the capillary. This method provides both qualitative and quantitative data of inorganic cations, with quantitation in zucchini, mushroom and apple samples in agreement with Sector Field Inductively Coupled Plasma Mass Spectrometric analysis (r2 = 0.98, n = 9). This method provides a new way for rapid, quantitative analysis by eliminating sample preparation procedures, and has great potential for a range of applications in plant science and food chemistry.

Funding

Australian Research Council

History

Publication title

Journal of Chromatography A

Volume

1428

Pagination

346-351

ISSN

0021-9673

Department/School

School of Natural Sciences

Publisher

Elsevier BV

Place of publication

Netherlands

Rights statement

© 2015 Elsevier

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the chemical sciences

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