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Direct electrokinetic injection of inorganic cations from whole fruits and vegetables for capillary electrophoresis analysis

Citation

Kalsoom, U and Guijt, RM and Boyce, MC and Townsend, AT and Haselberg, R and Breadmore, MC, Direct electrokinetic injection of inorganic cations from whole fruits and vegetables for capillary electrophoresis analysis, Journal of Chromatography A, 1428 pp. 346-351. ISSN 0021-9673 (2016) [Refereed Article]

Copyright Statement

© 2015 Elsevier

DOI: doi:10.1016/j.chroma.2015.08.012

Abstract

A novel approach for the direct injection from plant tissues without any sample pre-treatment has been developed by simply placing a small piece of the tissue into a capillary electrophoresis vial followed by application of a voltage for electrokinetic injection. Separations of sodium, potassium, calcium and magnesium were achieved using a BGE comprising 10 mM imidazole and 2.5 mM 18-crown-6-ether at pH 4.5. The addition of 2% (m/v) hydroxypropylmethyl cellulose to the separation buffer allowed for precise and accurate electrokinetic injection of ions from the plant material by halting the movement of tissue fluid into the capillary. This method provides both qualitative and quantitative data of inorganic cations, with quantitation in zucchini, mushroom and apple samples in agreement with Sector Field Inductively Coupled Plasma Mass Spectrometric analysis (r2 = 0.98, n = 9). This method provides a new way for rapid, quantitative analysis by eliminating sample preparation procedures, and has great potential for a range of applications in plant science and food chemistry.

Item Details

Item Type:Refereed Article
Keywords:electrophoresis, fruit, direct injection, plant tissue
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Kalsoom, U (Ms Umme Kalsoom)
Author:Guijt, RM (Dr Rosanne Guijt)
Author:Townsend, AT (Associate Professor Ashley Townsend)
Author:Breadmore, MC (Professor Michael Breadmore)
ID Code:108966
Year Published:2016
Funding Support:Australian Research Council (DP0984745)
Deposited By:Chemistry
Deposited On:2016-05-13
Last Modified:2018-03-14
Downloads:0

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