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Contrasting roles of condensin I and condensin II in mitotic chromosome formation

Citation

Green, LC and Kalitsis, P and Chang, TM and Cipetic, M and Kim, H and Marshall, O and Turnbull, L and Whitchurch, CB and Vagnarelli, P and Samejima, K and Earnshaw, WC and Choo, KHA and Hudson, DF, Contrasting roles of condensin I and condensin II in mitotic chromosome formation, Journal of Cell Science, 125, (6) pp. 1591-1604. ISSN 0021-9533 (2012) [Refereed Article]

DOI: doi:10.1242/jcs.097790

Abstract

In vertebrates, two condensin complexes exist, condensin I and condensin II, which have differing but unresolved roles in organizing mitotic chromosomes. To dissect accurately the role of each complex in mitosis, we have made and studied the first vertebrate conditional knockouts of the genes encoding condensin I subunit CAP-H and condensin II subunit CAP-D3 in chicken DT40 cells. Live-cell imaging reveals highly distinct segregation defects. CAP-D3 (condensin II) knockout results in masses of chromatin-containing anaphase bridges. CAP-H (condensin I)-knockout anaphases have a more subtle defect, with chromatids showing fine chromatin fibres that are associated with failure of cytokinesis and cell death. Super-resolution microscopy reveals that condensin-I-depleted mitotic chromosomes are wider and shorter, with a diffuse chromosome scaffold, whereas condensin-II-depleted chromosomes retain a more defined scaffold, with chromosomes more stretched and seemingly lacking in axial rigidity. We conclude that condensin II is required primarily to provide rigidity by establishing an initial chromosome axis around which condensin I can arrange loops of chromatin.

Item Details

Item Type:Refereed Article
Keywords:Condensin, Chromosome structure
Research Division:Biological Sciences
Research Group:Biochemistry and Cell Biology
Research Field:Biochemistry and Cell Biology not elsewhere classified
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Medical and Health Sciences
Author:Marshall, O (Dr Owen Marshall)
ID Code:108956
Year Published:2012
Web of Science® Times Cited:59
Deposited By:Menzies Institute for Medical Research
Deposited On:2016-05-12
Last Modified:2017-11-06
Downloads:0

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