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Active transcription and essential role of RNA polymerase II at the centromere during mitosis

Citation

Chan, FL and Marshall, OJ and Saffery, R and Kim, BW and Earle, E and Choo, KH and Wong, LH, Active transcription and essential role of RNA polymerase II at the centromere during mitosis, Proceedings of the National Academy of Sciences of The United States of America, 109, (6) pp. 1979-1984. ISSN 0027-8424 (2012) [Refereed Article]

DOI: doi:10.1073/pnas.1108705109

Abstract

Transcription of the centromeric regions has been reported to occur in G1 and S phase in different species. Here, we investigate whether transcription also occurs and plays a functional role at the mammalian centromere during mitosis. We show the presence of actively transcribing RNA polymerase II (RNAPII) and its associated transcription factors, coupled with the production of centromere satellite transcripts at the mitotic kinetochore. Specific inhibition of RNAPII activity during mitosis leads to a decrease in centromeric α-satellite transcription and a concomitant increase in anaphase-lagging cells, with the lagging chromosomes showing reduced centromere protein C binding. These findings demonstrate an essential role of RNAPII in the transcription of α-satellite DNA, binding of centromere protein C, and the proper functioning of the mitotic kinetochore.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Biochemistry and Cell Biology
Research Field:Biochemistry and Cell Biology not elsewhere classified
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Medical and Health Sciences
Author:Marshall, OJ (Dr Owen Marshall)
ID Code:108955
Year Published:2012
Web of Science® Times Cited:92
Deposited By:Menzies Institute for Medical Research
Deposited On:2016-05-12
Last Modified:2017-11-06
Downloads:0

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