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Active transcription and essential role of RNA polymerase II at the centromere during mitosis
journal contribution
posted on 2023-05-18, 19:27 authored by Chan, FL, Owen MarshallOwen Marshall, Saffery, R, Kim, BW, Earle, E, Choo, KH, Wong, LHTranscription of the centromeric regions has been reported to occur in G1 and S phase in different species. Here, we investigate whether transcription also occurs and plays a functional role at the mammalian centromere during mitosis. We show the presence of actively transcribing RNA polymerase II (RNAPII) and its associated transcription factors, coupled with the production of centromere satellite transcripts at the mitotic kinetochore. Specific inhibition of RNAPII activity during mitosis leads to a decrease in centromeric α-satellite transcription and a concomitant increase in anaphase-lagging cells, with the lagging chromosomes showing reduced centromere protein C binding. These findings demonstrate an essential role of RNAPII in the transcription of α-satellite DNA, binding of centromere protein C, and the proper functioning of the mitotic kinetochore.
History
Publication title
Proceedings of the National Academy of Sciences of The United States of AmericaVolume
109Issue
6Pagination
1979-1984ISSN
0027-8424Department/School
Menzies Institute for Medical ResearchPublisher
Natl Acad SciencesPlace of publication
2101 Constitution Ave Nw, Washington, USA, Dc, 20418Repository Status
- Restricted