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Mitogen activated Tasmanian devil blood mononuclear cells kill devil facial tumour disease cells

Citation

Brown, GK and Tovar, C and Cooray, AA and Kreiss, A and Darby, J and Murphy, JM and Corcoran, LM and Bettiol, SS and Lyons, AB and Woods, GM, Mitogen activated Tasmanian devil blood mononuclear cells kill devil facial tumour disease cells, Immunology and Cell Biology, 94, (7) pp. 673-679. ISSN 0818-9641 (2016) [Refereed Article]

Copyright Statement

Copyright 2016 Australasian Society for Immunology

DOI: doi:10.1038/icb.2016.38

Abstract

Devil Facial Tumour Disease (DFTD) is a transmissible cancer that has brought the host species, the Tasmanian devil, to the brink of extinction. The cancer cells avoid allogeneic immune recognition by down-regulating cell surface MHC I expression. This should prevent CD8+ T cell, but not NK cell, cytotoxicity. The reason why NK cells, normally reactive to MHC-negative cells, are not activated to kill DFTD cells has not been determined. The immune response of wild devils to DFTD, if it occurs, is uncharacterised. To investigate this, we tested twelve wild devils with DFTD, and found suggestive evidence of low levels of antibodies against DFTD cells in one devil. Eight of these devils were also analysed for cytotoxicity, however, none showed evidence for cytotoxicity against cultured DFTD cells. To establish whether mimicking activation of anti-tumour responses could induce cytotoxic activity against DFTD, Tasmanian devil peripheral blood mononuclear cells (PBMC) were treated with either the mitogen Concanavalin A, the toll-like receptor (TLR) agonist Poly I:C, or recombinant Tasmanian devil IL-2. All induced the PBMC cells to kill cultured DFTD cells, suggesting that activation does not occur after encounter with DFTD cells in vivo, but can be induced. The identification of agents that activate cytotoxicity against DFTD target cells is critical for developing strategies to protect against DFTD. Such agents could function as adjuvants to induce functional immune responses capable of targeting DFTD cells and tumours in vivo.

Item Details

Item Type:Refereed Article
Keywords:Devil Facial Tumour Disease, mitogen, mononuclear
Research Division:Medical and Health Sciences
Research Group:Medical Microbiology
Research Field:Medical Parasitology
Objective Division:Health
Objective Group:Other Health
Objective Field:Health not elsewhere classified
Author:Brown, GK (Ms Gabriella Brown)
Author:Tovar, C (Dr Cesar Tovar Lopez)
Author:Cooray, AA (Ms Anne Cooray)
Author:Kreiss, A (Dr Alexandre Kreiss)
Author:Darby, J (Ms Jocelyn Darby)
Author:Bettiol, SS (Dr Silvana Bettiol)
Author:Lyons, AB (Dr Bruce Lyons)
Author:Woods, GM (Professor Gregory Woods)
ID Code:108938
Year Published:2016
Web of Science® Times Cited:3
Deposited By:Medicine (Discipline)
Deposited On:2016-05-12
Last Modified:2017-08-02
Downloads:0

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