Chen, L and Wilson, R and Bennett, E and Zosky, GR, Identification of vitamin D sensitive pathways during lung development, Respiratory Research, 17 pp. 1-11. ISSN 1465-993X (2016) [Refereed Article]
© 2016 Chen et al. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
Background: We have previously shown that vitamin D deficiency has a detrimental impact on lung development. In this study, we aimed to identify the mechanisms linking vitamin D with lung development using a mouse model of dietary manipulation.
Methods: Female offspring were euthanized at different time-points; embryonic day (E)14.5, E17.5 or postnatal day (P)7. Lung tissue was collected for mass spectrometry-based proteomic analysis. Label-free quantitation was used to identify the differentially expressed proteins and ELISA confirmed the expression of selected proteins. Lungs from separate groups of mice were fixed and processed for stereological assessment of lung structure.
Results: No differences in protein expression between vitamin D deficient and replete mice were detected at E14.5 and E17.5, whereas 66 proteins were differentially expressed in P7 lungs. The expression of pulmonary surfactant-associated protein B (SP-B) and peroxiredoxin 5 (PRDX5) were reduced in P7 lungs of vitamin D deficient mice, while the production of collagen type Ι alpha 1 (COL1A1) was higher in lungs of vitamin D deficient mice. There were no differences in lung volume, parenchymal volume, volume of airspaces or surface area of airspaces between vitamin D deficient and vitamin D replete mice across three time-points.
Conclusions: The difference in protein expression during the early postnatal time-point suggests that vitamin D deficiency may induce alterations of lung structure and function in later life during alveolarization stage through impaired pulmonary surfactant production and anti-oxidative stress ability as well as enhanced collagen synthesis. These data provided a plausible mechanism linking maternal vitamin D deficiency with altered postnatal lung function.
|Item Type:||Refereed Article|
|Keywords:||vitamin D, lung development, vitamin D deficiency, proteomic mass spectrometry, pulmonary surfactant-associated protein B, collagen type Ι alpha 1, lung development|
|Research Division:||Biomedical and Clinical Sciences|
|Research Group:||Cardiovascular medicine and haematology|
|Research Field:||Respiratory diseases|
|Objective Group:||Clinical health|
|Objective Field:||Clinical health not elsewhere classified|
|UTAS Author:||Chen, L (Dr Ling Chen)|
|UTAS Author:||Wilson, R (Dr Richard Wilson)|
|UTAS Author:||Bennett, E (Dr Ellen Bennett)|
|UTAS Author:||Zosky, GR (Professor Graeme Zosky)|
|Funding Support:||National Health and Medical Research Council (1042235)|
|Web of Science® Times Cited:||29|
|Downloads:||139 View Download Statistics|
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