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TGF-beta receptor type II and fetuin in the developing sheep neocortex

Citation

Dziegielewska, KM and Williams, RT and Kitchener, PD and Knott, GW and Monk, SE and Potter, A and Saunders, NR, TGF-beta receptor type II and fetuin in the developing sheep neocortex, Cell and Tissue Research, 290, (3) pp. 515-524. ISSN 0302-766X (1997) [Refereed Article]

DOI: doi:10.1007/s004410050958

Abstract

Fetuin shows a characteristic pattern of distribution in the developing neocortex in many mammalian species. Its expression is confined to early-appearing cortical-plate and later subplate neurons. A short 19 aminoacid sequence of fetuin shows a degree of homology to an 18 amino-acid sequence of the TGF-β type II receptor (TβR-II) and in vitro fetuin binds to members of the TGF-β family of cytokines. It has been suggested that fetuin is the biologically significant antagonist of these cytokines. We have compared, using immunocytochemistry, the distribution pattern of TβR-II and fetuin in the developing neocortex of foetal sheep. TβR-II immunoreactivity first appears at around 40 days of gestation in the fetal sheep (E40, term in sheep is 150 days from conception), localised in two discreet bands: one just outside the cortical plate in the inner part of the marginal zone and one deep in the cortical plate in what becomes the transient subplate zone. By E70-E80, TβR-II is prominent in a population of subplate cells, whereas, by E120 only small patches of TβR-II-positive cells are visible, principally in pyramidal cells in layer VI. The developmental sequence of the staining pattern for TβR-II in the neocortex is complementary to that for fetuin, rather than overlapping with it. Double-labelling of fetuin and TβR-II shows some cellular co-localisation, especially at E60, but most fetuin-positive cells are not immunoreactive for TβR-II. Thus, fetuin's proposed role as an antagonist of TGF-β cytokines and mimic of TβR-II is not consistent with the observed distribution of these two molecules in the developing neocortex of the foetal sheep.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Neurosciences
Research Field:Cellular Nervous System
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Environmental Sciences
Author:Dziegielewska, KM (Dr Kate Dziegielewska)
Author:Kitchener, PD (Mr Peter Kitchener)
Author:Knott, GW (Dr Graham Knott)
Author:Monk, SE (Mrs Monk)
Author:Potter, A (Mrs Ann Potter)
Author:Saunders, NR (Professor Norman Saunders)
ID Code:10855
Year Published:1997
Web of Science® Times Cited:9
Deposited By:Anatomy and Physiology
Deposited On:1997-08-01
Last Modified:2011-08-11
Downloads:0

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